Nano Eggshell-titanium-dioxide Biocomposite in Occluding Opened Dentine Tubules

Overview

Journal Bioinformation

Specialty Biology

Date 2023 Sep 1

PMID 37654822

Authors

Navjot Singh Mann

Ashu Jhamb

Nimrat Kaur

Navneet Mann

Affiliations

Soon will be listed here.

Abstract

It is of interest to synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; and Group 4: GIC mousse. These were then, agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-treatment and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.

References

Pandit N, Gupta R, Bansal A . Comparative evaluation of two commercially available desensitizing agents for the treatment of dentinal hypersensitivity. Indian J Dent Res. 2013; 23(6):778-83. DOI: 10.4103/0970-9290.111259. View

Yang Z, Wang F, Lu K, Li Y, Zhou Z . Arginine-containing desensitizing toothpaste for the treatment of dentin hypersensitivity: a meta-analysis. Clin Cosmet Investig Dent. 2016; 8:1-14. PMC: 4708190. DOI: 10.2147/CCIDE.S95660. View

Schiff T, Delgado E, Zhang Y, Cummins D, DeVizio W, Mateo L . Clinical evaluation of the efficacy of an in-office desensitizing paste containing 8% arginine and calcium carbonate in providing instant and lasting relief of dentin hypersensitivity. Am J Dent. 2009; 22 Spec No A:8A-15A. View

Yu J, Yang H, Li K, Lei J, Zhou L, Huang C . A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: An in vitro study. J Dent. 2016; 50:21-9. DOI: 10.1016/j.jdent.2016.04.005. View

Yazicioglu O, Ulukapi H . The investigation of non-invasive techniques for treating early approximal carious lesions: an in vivo study. Int Dent J. 2014; 64(1):1-11. PMC: 9375040. DOI: 10.1111/idj.12056. View

Yassen G, Platt J, Hara A . Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci. 2011; 53(3):273-82. DOI: 10.2334/josnusd.53.273. View

Fonseca R, Haiter-Neto F, Fernandes-Neto A, Barbosa G, Soares C . Radiodensity of enamel and dentin of human, bovine and swine teeth. Arch Oral Biol. 2004; 49(11):919-22. DOI: 10.1016/j.archoralbio.2004.05.006. View

. Consensus-based recommendations for the diagnosis and management of dentin hypersensitivity. J Can Dent Assoc. 2003; 69(4):221-6. View

Cunha-Cruz J, Stout J, Heaton L, Wataha J . Dentin hypersensitivity and oxalates: a systematic review. J Dent Res. 2010; 90(3):304-10. PMC: 3144108. DOI: 10.1177/0022034510389179. View

10.

Colombo M, Mirando M, Rattalino D, Beltrami R, Chiesa M, Poggio C . Remineralizing effect of a zinc-hydroxyapatite toothpaste on enamel erosion caused by soft drinks: Ultrastructural analysis. J Clin Exp Dent. 2017; 9(7):e861-e868. PMC: 5553346. DOI: 10.4317/jced.53790. View

11.

Onwubu S, Vahed A, Singh S, Kanny K . Physicochemical characterization of a dental eggshell powder abrasive material. J Appl Biomater Funct Mater. 2017; 15(4):e341-e346. DOI: 10.5301/jabfm.5000361. View

12.

Saeki K, Marshall G, Gansky S, Parkinson C, Marshall S . Strontium effects on root dentin tubule occlusion and nanomechanical properties. Dent Mater. 2016; 32(2):240-51. DOI: 10.1016/j.dental.2015.11.020. View

13.

Nakashima S, Yoshie M, Sano H, Bahar A . Effect of a test dentifrice containing nano-sized calcium carbonate on remineralization of enamel lesions in vitro. J Oral Sci. 2009; 51(1):69-77. DOI: 10.2334/josnusd.51.69. View

14.

Taha S, Han H, Chang S, Sovadinova I, Kuroda K, Langford R . Nano/micro fluorhydroxyapatite crystal pastes in the treatment of dentin hypersensitivity: an in vitro study. Clin Oral Investig. 2015; 19(8):1921-30. DOI: 10.1007/s00784-015-1427-2. View

15.

Tanaka J, Medici Filho E, Salgado J, Salgado M, de Moraes L, Moraes M . Comparative analysis of human and bovine teeth: radiographic density. Braz Oral Res. 2009; 22(4):346-51. DOI: 10.1590/s1806-83242008000400011. View

16.

Wang Z, Sa Y, Sauro S, Chen H, Xing W, Ma X . Effect of desensitising toothpastes on dentinal tubule occlusion: a dentine permeability measurement and SEM in vitro study. J Dent. 2010; 38(5):400-10. DOI: 10.1016/j.jdent.2010.01.007. View

17.

Pashley D, OMeara J, Kepler E, Galloway S, Thompson S, Stewart F . Dentin permeability. Effects of desensitizing dentifrices in vitro. J Periodontol. 1984; 55(9):522-5. DOI: 10.1902/jop.1984.55.9.522. View

18.

Poggio C, Gulino C, Mirando M, Colombo M, Pietrocola G . Protective effect of zinc-hydroxyapatite toothpastes on enamel erosion: An study. J Clin Exp Dent. 2017; 9(1):e118-e122. PMC: 5268095. DOI: 10.4317/jced.53068. View

19.

Arnold W, Prange M, Naumova E . Effectiveness of various toothpastes on dentine tubule occlusion. J Dent. 2015; 43(4):440-9. DOI: 10.1016/j.jdent.2015.01.014. View

20.

Costa B, Iwamoto A, Puppin-Rontani R, Pascon F . Comparative Analysis of Root Dentin Morphology and Structure of Human Versus Bovine Primary Teeth. Microsc Microanal. 2015; 21(3):689-94. DOI: 10.1017/S1431927615000434. View