Neuroprotective Effects of Goji Berry (Lycium Barbarum L.) Polysaccharides on Depression-like Behavior in Ovariectomized Rats: Behavioral and Biochemical Evidence

Overview

Journal Croat Med J

Specialty General Medicine

Date 2023 Sep 1

PMID 37654035

Authors

Hayriye Soyturk

Bihter Gokce Bozat

Fatma Pehlivan Karakas

Hamit Coskun

Tulin Firat

Affiliations

Soon will be listed here.

Abstract

Aim: To assess the protective effects of goji berry (Lycium barbarum L.) polysaccharides (LBP) on depression-like behavior in ovariectomized rats and to elucidate the mechanisms underlying these effects.

Methods: One hundred female Wistar albino rats (three months old) were randomly assigned either to ovariectomy (n=50) or sham surgery (n=50). After a 14-day recovery period, the groups were divided into five treatment subgroups (10 per group): high-dose LBP (200 mg/kg), low-dose LBP (20 mg/kg), imipramine (IMP, 2.5 mg/kg), 17-beta estradiol (E2, 1 mg/kg), and distilled water. Then, rats underwent a forced swimming test. We also determined the levels of serum antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde), E2 levels, hippocampal brain-derived neurotrophic factor (BDNF), 5HT2A receptor, and transferase dUTP nick end labeling (TUNEL)-positive cells.

Results: Both low-dose LBP and imipramine decreased depression-like behavior by increasing serum superoxide dismutase activity and by decreasing serum malondialdehyde level. Furthermore, low-dose LPB, high-dose LBP, and imipramine increased the number of 5-HT2A receptor- and BDNF-positive cells but decreased the number of TUNEL-positive cells in the hippocampus.

Conclusion: This is the first study to show the antidepressant effect of LBP. Although additional research is needed, LBP may be considered a potential new antidepressant.

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References

Bertrand P, Paranavitane U, Chavez C, Gogos A, Jones M, van den Buuse M . The effect of low estrogen state on serotonin transporter function in mouse hippocampus: a behavioral and electrochemical study. Brain Res. 2005; 1064(1-2):10-20. DOI: 10.1016/j.brainres.2005.10.018. View

Shah N, Wong T . Current breast cancer risks of hormone replacement therapy in postmenopausal women. Expert Opin Pharmacother. 2006; 7(18):2455-63. PMC: 2670363. DOI: 10.1517/14656566.7.18.2455. View

Bjorkholm C, Monteggia L . BDNF - a key transducer of antidepressant effects. Neuropharmacology. 2015; 102:72-9. PMC: 4763983. DOI: 10.1016/j.neuropharm.2015.10.034. View

Gatson J, Liu M, Abdelfattah K, Wigginton J, Smith S, Wolf S . Estrone is neuroprotective in rats after traumatic brain injury. J Neurotrauma. 2012; 29(12):2209-19. PMC: 3960846. DOI: 10.1089/neu.2011.2274. View

Bethea C, Reddy A, Tokuyama Y, Henderson J, Lima F . Protective actions of ovarian hormones in the serotonin system of macaques. Front Neuroendocrinol. 2009; 30(2):212-38. PMC: 2704571. DOI: 10.1016/j.yfrne.2009.04.003. View

Daubert E, Condron B . Serotonin: a regulator of neuronal morphology and circuitry. Trends Neurosci. 2010; 33(9):424-34. PMC: 2929308. DOI: 10.1016/j.tins.2010.05.005. View

Rodriguez-Landa J . Considerations of Timing Post-ovariectomy in Mice and Rats in Studying Anxiety- and Depression-Like Behaviors Associated With Surgical Menopause in Women. Front Behav Neurosci. 2022; 16:829274. PMC: 8931748. DOI: 10.3389/fnbeh.2022.829274. View

Lanctot K, Herrmann N, Mazzotta P . Role of serotonin in the behavioral and psychological symptoms of dementia. J Neuropsychiatry Clin Neurosci. 2001; 13(1):5-21. DOI: 10.1176/jnp.13.1.5. View

Pokorski M, Siwiec P . Memory, depression, and progressing old age in women. J Physiol Pharmacol. 2006; 57 Suppl 4:297-304. View

10.

Cominski T, Jiao X, Catuzzi J, Stewart A, Pang K . The role of the hippocampus in avoidance learning and anxiety vulnerability. Front Behav Neurosci. 2014; 8:273. PMC: 4125878. DOI: 10.3389/fnbeh.2014.00273. View

11.

Pehlivan Karakas F . Efficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulators. Plant Physiol Biochem. 2019; 146:384-391. DOI: 10.1016/j.plaphy.2019.11.009. View

12.

Kim H, Lee Y, Kim T, Lim R, Hwang D, Moffat J . Asparagus cochinchinensis extract ameliorates menopausal depression in ovariectomized rats under chronic unpredictable mild stress. BMC Complement Med Ther. 2020; 20(1):325. PMC: 7590795. DOI: 10.1186/s12906-020-03121-0. View

13.

Abd-Rabo M, Georgy G, Saied N, Hassan W . Involvement of the serotonergic system and neuroplasticity in the antidepressant effect of curcumin in ovariectomized rats: Comparison with oestradiol and fluoxetine. Phytother Res. 2018; 33(2):387-396. DOI: 10.1002/ptr.6232. View

14.

Chandra Mondal A, Fatima M . Direct and indirect evidences of BDNF and NGF as key modulators in depression: role of antidepressants treatment. Int J Neurosci. 2018; 129(3):283-296. DOI: 10.1080/00207454.2018.1527328. View

15.

Li S, Yang D, Yeung C, Yu W, Chang R, So K . Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury. PLoS One. 2011; 6(1):e16380. PMC: 3027646. DOI: 10.1371/journal.pone.0016380. View

16.

Mann J . Role of the serotonergic system in the pathogenesis of major depression and suicidal behavior. Neuropsychopharmacology. 1999; 21(2 Suppl):99S-105S. DOI: 10.1016/S0893-133X(99)00040-8. View

17.

Pehlivan Karakas F, Coskun H, Soyturk H, Bozat B . Anxiolytic, antioxidant, and neuroprotective effects of goji berry polysaccharides in ovariectomized rats: experimental evidence from behavioral, biochemical, and immunohistochemical analyses. Turk J Biol. 2020; 44(5):238-251. PMC: 7585160. DOI: 10.3906/biy-2003-8. View

18.

Mandrioli R, Protti M, Mercolini L . New-Generation, Non-SSRI Antidepressants: Therapeutic Drug Monitoring and Pharmacological Interactions. Part 1: SNRIs, SMSs, SARIs. Curr Med Chem. 2017; 25(7):772-792. DOI: 10.2174/0929867324666170712165042. View

19.

Zhang Z, Hong J, Zhang S, Zhang T, Sha S, Yang R . Postpartum estrogen withdrawal impairs hippocampal neurogenesis and causes depression- and anxiety-like behaviors in mice. Psychoneuroendocrinology. 2016; 66:138-49. DOI: 10.1016/j.psyneuen.2016.01.013. View

20.

Malberg J, Duman R . Cell proliferation in adult hippocampus is decreased by inescapable stress: reversal by fluoxetine treatment. Neuropsychopharmacology. 2003; 28(9):1562-71. DOI: 10.1038/sj.npp.1300234. View