Effect of Leaf Powder on Hematological Profile of Male Wistar Rats

Overview

Journal J Blood Med

Publisher Dove Medical Press

Specialty Hematology

Date 2023 Aug 29

PMID 37641693

Authors

Titing Nurhayati

Muhammad Irfan Fathoni

Siti Nur Fatimah

Vita Murniati Tarawan

Hanna Goenawan

Resti Gradia Dwiwina

Affiliations

Soon will be listed here.

Abstract

Background: Indonesia is a country with high biodiversity of more than 20,000 plant species, and 35% of them are identified as having health benefits. is one plant that almost all of its parts have been used as nutritional supplements and traditional medicines. Moringa leaves contain nutrients, antioxidants, and bioactive substances that have anti-inflammatory, wound healing, and anti-anemia properties.

Purpose: This study aimed to investigate the hematological effect of Moringa leaf powder in male Wistar rats under normal conditions.

Methods: Twenty-four male Wistar rats strain () 9-10 weeks old and 250-275 grams were divided into four groups (n=6), normal as a control group and three other groups were given Moringa leaf powder at doses 200, 400, and 800 mg/kgBW during 12 weeks. Blood samples at week 12 were administered to determine blood count.

Results: The results of this study showed differences between the various doses of Moringa leaf powder for each hematological profile. These differences were more significant for MCH parameters that indicated a decrease in the D800 group compared with the control group.

Conclusion: In conclusion, this study revealed that the consumption of Moringa leaf powder for 12 weeks did not have a significant change in the hematological profile, except for the MCH value that revealed a modification.

Citing Articles

Putative effects of moringa oil or its nano-emulsion on the growth, physiological responses, blood health, semen quality, and the sperm antioxidant-related genes in ram.

Ismail R, Khalil W, Grawish S, Mahmoud K, Abdelnour S, Gad A BMC Vet Res. 2025; 21(1):11.

PMID: 39789573 PMC: 11715245. DOI: 10.1186/s12917-024-04444-7.

References

Salmeron-Manzano E, Garrido-Cardenas J, Manzano-Agugliaro F . Worldwide Research Trends on Medicinal Plants. Int J Environ Res Public Health. 2020; 17(10). PMC: 7277765. DOI: 10.3390/ijerph17103376. View

El-Dahiyat F, Rashrash M, Abuhamdah S, Farha R, Babar Z . Herbal medicines: a cross-sectional study to evaluate the prevalence and predictors of use among Jordanian adults. J Pharm Policy Pract. 2020; 13:2. PMC: 6971905. DOI: 10.1186/s40545-019-0200-3. View

Chin C, Jalil J, Ng P, Ng S . Development and formulation of Moringa oleifera standardised leaf extract film dressing for wound healing application. J Ethnopharmacol. 2017; 212:188-199. DOI: 10.1016/j.jep.2017.10.016. View

Kashyap P, Kumar S, Riar C, Jindal N, Baniwal P, Guine R . Recent Advances in Drumstick () Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary Applications. Antioxidants (Basel). 2022; 11(2). PMC: 8869219. DOI: 10.3390/antiox11020402. View

Ndong M, Uehara M, Katsumata S, Sato S, Suzuki K . Preventive effects of Moringa oleifera (Lam) on hyperlipidemia and hepatocyte ultrastructural changes in iron deficient rats. Biosci Biotechnol Biochem. 2007; 71(8):1826-33. DOI: 10.1271/bbb.60644. View

Martinez-Gonzalez C, Martinez L, Martinez-Ortiz E, Gonzalez-Trujano M, Deciga-Campos M, Ventura-Martinez R . Moringa oleifera, a species with potential analgesic and anti-inflammatory activities. Biomed Pharmacother. 2017; 87:482-488. DOI: 10.1016/j.biopha.2016.12.107. View

Pretini V, Koenen M, Kaestner L, Fens M, Schiffelers R, Bartels M . Red Blood Cells: Chasing Interactions. Front Physiol. 2019; 10:945. PMC: 6684843. DOI: 10.3389/fphys.2019.00945. View

Jacob Filho W, Lima C, Paunksnis M, Silva A, Perilhao M, Caldeira M . Reference database of hematological parameters for growing and aging rats. Aging Male. 2017; 21(2):145-148. DOI: 10.1080/13685538.2017.1350156. View

Holinstat M . Normal platelet function. Cancer Metastasis Rev. 2017; 36(2):195-198. PMC: 5709181. DOI: 10.1007/s10555-017-9677-x. View

10.

Mahdi H, Khan N, Asmawi M, Mahmud R, AL Murugaiyah V . anti-arthritic and anti-nociceptive effects of ethanol extract of leaves on complete Freund's adjuvant (CFA)-induced arthritis in rats. Integr Med Res. 2018; 7(1):85-94. PMC: 5884001. DOI: 10.1016/j.imr.2017.11.002. View

11.

Kim J, Kismali G, Gupta S . Natural Products for the Prevention and Treatment of Chronic Inflammatory Diseases: Integrating Traditional Medicine into Modern Chronic Diseases Care. Evid Based Complement Alternat Med. 2018; 2018:9837863. PMC: 5899861. DOI: 10.1155/2018/9837863. View

12.

Lesjak M, Hoque R, Balesaria S, Skinner V, Debnam E, Srai S . Quercetin inhibits intestinal iron absorption and ferroportin transporter expression in vivo and in vitro. PLoS One. 2014; 9(7):e102900. PMC: 4109952. DOI: 10.1371/journal.pone.0102900. View

13.

OConnell K, Mikkola A, Stepanek A, Vernet A, Hall C, Sun C . Practical murine hematopathology: a comparative review and implications for research. Comp Med. 2015; 65(2):96-113. PMC: 4408895. View

14.

Chiabrando D, Mercurio S, Tolosano E . Heme and erythropoieis: more than a structural role. Haematologica. 2014; 99(6):973-83. PMC: 4040894. DOI: 10.3324/haematol.2013.091991. View

15.

Fujii T, Asai T, Matsuo T, Okamura K . Effect of resistance exercise on iron status in moderately iron-deficient rats. Biol Trace Elem Res. 2011; 144(1-3):983-91. DOI: 10.1007/s12011-011-9072-3. View

16.

Jain A, Jain S, Singh N, Aswal P, Pal S, Meinia S . Storage stability of commonly used haematological parameters at 33 °C: Electronic supplementary material available online for this article. Biochem Med (Zagreb). 2018; 28(2):020901. PMC: 5898956. DOI: 10.11613/BM.2018.020901. View