A Unique Poly(A) Tail Profile Uncovers the Stability and Translational Activation of TOP Transcripts During Neuronal Differentiation

Overview

Journal iScience

Publisher Cell Press

Date 2023 Aug 28

PMID 37636056

Authors

Marine Baptissart

Brian N Papas

Ru-Pin Alicia Chi

Yin Li

Dongwon Lee

Bhairavy Puviindran

Marcos Morgan

Affiliations

Soon will be listed here.

Abstract

Cell differentiation is associated with global changes in translational activity. Here, we characterize how mRNA poly(A) tail processing supports this dynamic. We observe that decreased translation during neuronal differentiation of P19 cells correlates with the downregulation of 5'-terminal oligopyrimidine (TOP) transcripts which encode the translational machinery. Despite their downregulation, TOP transcripts remain highly stable and show increased translation as cells differentiate. Changes in TOP mRNA metabolism are reflected by their accumulation with poly(A) tails ∼60-nucleotide (nt) long. The dynamic changes in poly(A) processing can be partially recapitulated by depleting LARP1 or activating the mTOR pathway in undifferentiated cells. Although mTOR-induced accumulation of TOP mRNAs with tails ∼60-nt long does not trigger differentiation, it is associated with reduced proliferation of neuronal progenitors. We propose that while TOP mRNAs are transcriptionally silenced, their post-transcriptional regulation mediated by a specific poly(A) processing ensures an adequate supply of ribosomes to complete differentiation.

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