Pulmonary Delivery of Mucus-traversing PF127-modified Silk Fibroin Nanoparticles Loading with Quercetin for Lung Cancer Therapy

Overview

Journal Asian J Pharm Sci

Date 2023 Aug 28

PMID 37635802

Authors

Yu Tang

Lanfang Zhang

Rui Sun

Baiyi Luo

Yu Zhou

Yan Zhang

Yuqi Liang

Bo Xiao

Chenhui Wang

Affiliations

Soon will be listed here.

Abstract

The mucosal barrier remains a major barrier in the pulmonary drug delivery system, as mucociliary clearance in the airway accelerates the removal of inhaled nanoparticles (NPs). Herein, we designed and developed the inhalable Pluronic F127-modified silk fibroin NPs loading with quercetin (marked as QR-SF (PF127) NPs), aiming to solve the airway mucus barrier and improve the cancer therapeutic effect of QR. The PF127 coating on the SF NPs could attenuate the interaction between NPs and mucin proteins, thus facilitating the diffusion of SF(PF127) NPs in the mucus layer. The QR-SF (PF127) NPs had particle sizes of approximately 200 nm with negatively charged surfaces and showed constant drug release properties. Fluorescence recovery after photobleaching (FRAP) assay and transepithelial transport test showed that QR-SF (PF127) NPs exhibited superior mucus-penetrating ability in artificial mucus and monolayer Calu-3 cell model. Notably, a large amount of QR-SF (PF127) NPs distributed uniformly in the mice airway section, indicating the good retention of NPs in the respiratory tract. The mice melanoma lung metastasis model was established, and the therapeutic effect of QR-SF (PF127) NPs was significantly improved . PF127-modified SF NPs may be a promising strategy to attenuate the interaction with mucin proteins and enhance mucus penetration efficiency in the pulmonary drug delivery system.

Citing Articles

Inhaled Ivermectin-Loaded Lipid Polymer Hybrid Nanoparticles: Development and Characterization.

Kassaee S, Ayoko G, Richard D, Wang T, Islam N Pharmaceutics. 2024; 16(8).

PMID: 39204406 PMC: 11359515. DOI: 10.3390/pharmaceutics16081061.

Quercetin in Oncology: A Phytochemical with Immense Therapeutic Potential.

Kamal R, Paul P, Thakur S, Singh S, Awasthi A Curr Drug Targets. 2024; 25(11):740-751.

PMID: 38988154 DOI: 10.2174/0113894501292466240627050638.

Nanocarrier-Mediated Drug Delivery via Inhalational Route for Lung Cancer Therapy: A Systematic and Updated Review.

Ara N, Hafeez A AAPS PharmSciTech. 2024; 25(3):47.

PMID: 38424367 DOI: 10.1208/s12249-024-02758-1.

References

Vinayak M, Maurya A . Quercetin Loaded Nanoparticles in Targeting Cancer: Recent Development. Anticancer Agents Med Chem. 2019; 19(13):1560-1576. DOI: 10.2174/1871520619666190705150214. View

Ekstrom A, Serafini M, Nyren O, Wolk A, Bosetti C, Bellocco R . Dietary quercetin intake and risk of gastric cancer: results from a population-based study in Sweden. Ann Oncol. 2010; 22(2):438-43. PMC: 3030468. DOI: 10.1093/annonc/mdq390. View

Liu W, Zhou Z, Zhang S, Shi Z, Tabarini J, Lee W . Precise Protein Photolithography (P): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist. Adv Sci (Weinh). 2017; 4(9):1700191. PMC: 5604371. DOI: 10.1002/advs.201700191. View

Lv S, Tang Z, Li M, Lin J, Song W, Liu H . Co-delivery of doxorubicin and paclitaxel by PEG-polypeptide nanovehicle for the treatment of non-small cell lung cancer. Biomaterials. 2014; 35(23):6118-29. DOI: 10.1016/j.biomaterials.2014.04.034. View

Zhou Q, Leung S, Tang P, Parumasivam T, Loh Z, Chan H . Inhaled formulations and pulmonary drug delivery systems for respiratory infections. Adv Drug Deliv Rev. 2014; 85:83-99. DOI: 10.1016/j.addr.2014.10.022. View

Kumbhar P, Manjappa A, Shah R, Jha N, Singh S, Dua K . Inhalation delivery of repurposed drugs for lung cancer: Approaches, benefits and challenges. J Control Release. 2021; 341:1-15. DOI: 10.1016/j.jconrel.2021.11.015. View

Xu P, Kankala R, Pan Y, Yuan H, Wang S, Chen A . Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology. Int J Nanomedicine. 2018; 13:4685-4698. PMC: 6103603. DOI: 10.2147/IJN.S169399. View

Yue P, Zhou W, Huang G, Lei F, Chen Y, Ma Z . Nanocrystals based pulmonary inhalation delivery system: advance and challenge. Drug Deliv. 2022; 29(1):637-651. PMC: 8865109. DOI: 10.1080/10717544.2022.2039809. View

Ju Y, Cortez-Jugo C, Chen J, Wang T, Mitchell A, Tsantikos E . Engineering of Nebulized Metal-Phenolic Capsules for Controlled Pulmonary Deposition. Adv Sci (Weinh). 2020; 7(6):1902650. PMC: 7080547. DOI: 10.1002/advs.201902650. View

10.

Mottaghitalab F, Kiani M, Farokhi M, Kundu S, Reis R, Gholami M . Targeted Delivery System Based on Gemcitabine-Loaded Silk Fibroin Nanoparticles for Lung Cancer Therapy. ACS Appl Mater Interfaces. 2017; 9(37):31600-31611. DOI: 10.1021/acsami.7b10408. View

11.

Li X, Chen D, Le C, Zhu C, Gan Y, Hovgaard L . Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake. Int J Nanomedicine. 2011; 6:3151-62. PMC: 3235033. DOI: 10.2147/IJN.S25741. View

12.

Pellosi D, dAngelo I, Maiolino S, Mitidieri E, dEmmanuele di Villa Bianca R, Sorrentino R . In vitro/in vivo investigation on the potential of Pluronic® mixed micelles for pulmonary drug delivery. Eur J Pharm Biopharm. 2018; 130:30-38. DOI: 10.1016/j.ejpb.2018.06.006. View

13.

Mehnert W, Mader K . Solid lipid nanoparticles: production, characterization and applications. Adv Drug Deliv Rev. 2001; 47(2-3):165-96. DOI: 10.1016/s0169-409x(01)00105-3. View

14.

Dames P, Gleich B, Flemmer A, Hajek K, Seidl N, Wiekhorst F . Targeted delivery of magnetic aerosol droplets to the lung. Nat Nanotechnol. 2008; 2(8):495-9. DOI: 10.1038/nnano.2007.217. View

15.

Xu Q, Boylan N, Cai S, Miao B, Patel H, Hanes J . Scalable method to produce biodegradable nanoparticles that rapidly penetrate human mucus. J Control Release. 2013; 170(2):279-86. PMC: 3730524. DOI: 10.1016/j.jconrel.2013.05.035. View

16.

Zhao Z, Li Y, Xie M . Silk fibroin-based nanoparticles for drug delivery. Int J Mol Sci. 2015; 16(3):4880-903. PMC: 4394455. DOI: 10.3390/ijms16034880. View

17.

Zhang X, Huang Y, Song H, Canup B, Gou S, She Z . Inhibition of growth and lung metastasis of breast cancer by tumor-homing triple-bioresponsive nanotherapeutics. J Control Release. 2020; 328:454-469. DOI: 10.1016/j.jconrel.2020.08.066. View

18.

Hansson G . Mucus and mucins in diseases of the intestinal and respiratory tracts. J Intern Med. 2019; 285(5):479-490. PMC: 6497544. DOI: 10.1111/joim.12910. View

19.

Leem J, Kim M, Choi S, Kim S, Kim S, Song Y . Edible unclonable functions. Nat Commun. 2020; 11(1):328. PMC: 6965141. DOI: 10.1038/s41467-019-14066-5. View

20.

Elzoghby A, Hemasa A, Freag M . Hybrid protein-inorganic nanoparticles: From tumor-targeted drug delivery to cancer imaging. J Control Release. 2016; 243:303-322. DOI: 10.1016/j.jconrel.2016.10.023. View