Plant Resistance Inducers Affect Multiple Epidemiological Components of on Grapevine Leaves

Overview

Journal Plants (Basel)

Date 2023 Aug 26

PMID 37631150

Authors

Othmane Taibi

Irene Salotti

Vittorio Rossi

Affiliations

Soon will be listed here.

Abstract

Plant resistance inducers (PRIs) harbor promising potential for use in downy mildew (DM) control in viticulture. Here, the effects of six commercial PRIs on some epidemiological components of (Pv) on grapevine leaves were studied over 3 years. Disease severity, mycelial colonization of leaf tissue, sporulation severity, production of sporangia on affected leaves, and per unit of DM lesion were evaluated by inoculating the leaves of PRI-treated plants at 1, 3, 6, 12, and 19 days after treatment (DAT). Laminarin, potassium phosphonate (PHO), and fosetyl-aluminium (FOS) were the most effective in reducing disease severity as well as the Pv DNA concentration of DM lesions on leaves treated and inoculated at 1 and 3 DAT; PHO and FOS also showed long-lasting effects on leaves established after treatment (inoculations at 6 to 19 DAT). PRIs also prevented the sporulation of Pv on lesions; all the PRI-treated leaves produced fewer sporangia than the nontreated control, especially in PHO-, FOS-, and cerevisane-treated leaves (>75% reduction). These results illustrate the broader and longer effect of PRIs on DM epidemics. The findings open up new perspectives for using PRIs in a defense program based on single, timely, and preventative field interventions.

References

Wilkinson S, Mageroy M, Lopez Sanchez A, Smith L, Furci L, Cotton T . Surviving in a Hostile World: Plant Strategies to Resist Pests and Diseases. Annu Rev Phytopathol. 2019; 57:505-529. DOI: 10.1146/annurev-phyto-082718-095959. View

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

Mohamed N, Lherminier J, Farmer M, Fromentin J, Beno N, Houot V . Defense Responses in Grapevine Leaves Against Botrytis cinerea Induced by Application of a Pythium oligandrum Strain or Its Elicitin, Oligandrin, to Roots. Phytopathology. 2008; 97(5):611-20. DOI: 10.1094/PHYTO-97-5-0611. View

Bleyer G, Losch F, Schumacher S, Fuchs R . Together for the Better: Improvement of a Model Based Strategy for Grapevine Downy Mildew Control by Addition of Potassium Phosphonates. Plants (Basel). 2020; 9(6). PMC: 7355483. DOI: 10.3390/plants9060710. View

Heloir M, Adrian M, Brule D, Claverie J, Cordelier S, Daire X . Recognition of Elicitors in Grapevine: From MAMP and DAMP Perception to Induced Resistance. Front Plant Sci. 2019; 10:1117. PMC: 6760519. DOI: 10.3389/fpls.2019.01117. View

Balestrini R, Ghignone S, Quiroga G, Fiorilli V, Romano I, Gambino G . Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine Nebbiolo on Berry Transcriptome. Int J Mol Sci. 2020; 21(17). PMC: 7504522. DOI: 10.3390/ijms21176067. View

Huot B, Yao J, Montgomery B, He S . Growth-defense tradeoffs in plants: a balancing act to optimize fitness. Mol Plant. 2014; 7(8):1267-1287. PMC: 4168297. DOI: 10.1093/mp/ssu049. View

Lakkis S, Trotel-Aziz P, Rabenoelina F, Schwarzenberg A, Nguema-Ona E, Clement C . Strengthening Grapevine Resistance by PTA-CT2 Relies on Distinct Defense Pathways in Susceptible and Partially Resistant Genotypes to Downy Mildew and Gray Mold Diseases. Front Plant Sci. 2019; 10:1112. PMC: 6759587. DOI: 10.3389/fpls.2019.01112. View

Jones J, Dangl J . The plant immune system. Nature. 2006; 444(7117):323-9. DOI: 10.1038/nature05286. View

10.

Mestre P, Arista G, Piron M, Rustenholz C, Ritzenthaler C, Merdinoglu D . Identification of a Vitis vinifera endo-β-1,3-glucanase with antimicrobial activity against Plasmopara viticola. Mol Plant Pathol. 2016; 18(5):708-719. PMC: 6638254. DOI: 10.1111/mpp.12431. View

11.

Manghi M, Masiol M, Calzavara R, Graziano P, Peruzzi E, Pavoni B . The use of phosphonates in agriculture. Chemical, biological properties and legislative issues. Chemosphere. 2021; 283:131187. DOI: 10.1016/j.chemosphere.2021.131187. View

12.

Aziz A, Poinssot B, Daire X, Adrian M, Bezier A, Lambert B . Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Mol Plant Microbe Interact. 2003; 16(12):1118-28. DOI: 10.1094/MPMI.2003.16.12.1118. View

13.

Martinez-Medina A, Flors V, Heil M, Mauch-Mani B, Pieterse C, Pozo M . Recognizing Plant Defense Priming. Trends Plant Sci. 2016; 21(10):818-822. DOI: 10.1016/j.tplants.2016.07.009. View

14.

Toffolatti S, Venturini G, Maffi D, Vercesi A . Phenotypic and histochemical traits of the interaction between Plasmopara viticola and resistant or susceptible grapevine varieties. BMC Plant Biol. 2012; 12:124. PMC: 3509031. DOI: 10.1186/1471-2229-12-124. View

15.

Allegre M, Heloir M, Trouvelot S, Daire X, Pugin A, Wendehenne D . Are grapevine stomata involved in the elicitor-induced protection against downy mildew?. Mol Plant Microbe Interact. 2009; 22(8):977-86. DOI: 10.1094/MPMI-22-8-0977. View

16.

Singh R, Chinnasri B, De Smet L, Haeck A, Demeestere K, Van Cutsem P . Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway. Plant Physiol Biochem. 2019; 142:202-210. DOI: 10.1016/j.plaphy.2019.07.003. View

17.

Bove F, Bavaresco L, Caffi T, Rossi V . Assessment of Resistance Components for Improved Phenotyping of Grapevine Varieties Resistant to Downy Mildew. Front Plant Sci. 2019; 10:1559. PMC: 6890843. DOI: 10.3389/fpls.2019.01559. View

18.

Nelson T, Lewis B . Separation and characterization of the soluble and insoluble components of insoluble laminaran. Carbohydr Res. 1974; 33(1):63-74. DOI: 10.1016/s0008-6215(00)82940-7. View

19.

Kennelly M, Gadoury D, Wilcox W, Magarey P, Seem R . Primary Infection, Lesion Productivity, and Survival of Sporangia in the Grapevine Downy Mildew Pathogen Plasmopara viticola. Phytopathology. 2008; 97(4):512-22. DOI: 10.1094/PHYTO-97-4-0512. View

20.

De Miccolis Angelini R, Rotolo C, Gerin D, Abate D, Pollastro S, Faretra F . Global transcriptome analysis and differentially expressed genes in grapevine after application of the yeast-derived defense inducer cerevisane. Pest Manag Sci. 2019; 75(7):2020-2033. DOI: 10.1002/ps.5317. View