Characterization and Antioxidant Activity of Exopolysaccharides Produced by Sp. Nov Isolated from the Root of L

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Aug 26

PMID 37630460

Authors

Inhyup Kim

Geeta Chhetri

Yoonseop So

Sunho Park

Yonghee Jung

Haejin Woo

Taegun Seo

Affiliations

Soon will be listed here.

Abstract

Microbial exopolysaccharides (EPSs) have attracted attention from several fields due to their high industrial applicability. In the present study, rhizosphere strain CJ11 was isolated from the root of L. in Goyang-si, Republic of Korea, and a novel exopolysaccharide was purified from the sp. CJ11 fermentation broth. The exopolysaccharide's average molecular weight was 0.93 × 10 Da. Its monosaccharide composition included 72.2% mannose, 17.2% glucose, 7.8% galactose, and 2.8% arabinose. Fourier-transform infrared spectroscopy identified the exopolysaccharide carbohydrate polymer functional groups, and the structural properties were investigated using nuclear magnetic resonance. In addition, a microstructure of lyophilized EPS was determined by scanning electron microscopy. Using thermogravimetric analysis, the degradation of the exopolysaccharide produced by strain CJ11 was determined to be 210 °C. The exopolysaccharide at a concentration of 4 mg/mL exhibited 2,2-diphenyl-1-picrylhydrazyl free-radical-scavenging activity of 73.47%. Phylogenetic analysis based on the 16S rRNA gene sequencing results revealed that strain CJ11 was a novel isolate for which the name sp. nov is proposed.

Citing Articles

Potential Bioactivities, Chemical Composition, and Conformation Studies of Exopolysaccharide-Derived sp. Strain GAD7.

Ibrahim M, Ibrahim H, Haga T, Ishida A, Nehira T, Matsuo K J Fungi (Basel). 2024; 10(9).

PMID: 39330418 PMC: 11432975. DOI: 10.3390/jof10090659.

Characterization of Lactic Acid Bacterium Exopolysaccharide, Biological, and Nutritional Evaluation of Probiotic Formulated Fermented Coconut Beverage.

Adebayo-Tayo B, Ogundele B, Ajani O, Olaniyi O Int J Food Sci. 2024; 2024:8923217.

PMID: 39257841 PMC: 11383652. DOI: 10.1155/2024/8923217.

A novel exopolysaccharide-producing bacterium, sp. nov. isolated from , enhances the growth of subsp. under drought and salinity stress.

Kim I, Woo H, Chhetri G, Park S, Seo T Front Plant Sci. 2024; 15:1417639.

PMID: 39081520 PMC: 11286387. DOI: 10.3389/fpls.2024.1417639.

Roseateles caseinilyticus sp. nov. and Roseateles cellulosilyticus sp. nov., isolated from rice paddy field soil.

So Y, Chhetri G, Kim I, Park S, Jung Y, Seo T Antonie Van Leeuwenhoek. 2024; 117(1):87.

PMID: 38833203 DOI: 10.1007/s10482-024-01988-4.

Validation List no. 217. Valid publication of new names and new combinations effectively published outside the IJSEM.

Oren A, Goker M Int J Syst Evol Microbiol. 2024; 74(5).

PMID: 38828845 PMC: 11165881. DOI: 10.1099/ijsem.0.006275.

References

Overbeek R, Olson R, Pusch G, Olsen G, Davis J, Disz T . The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 2013; 42(Database issue):D206-14. PMC: 3965101. DOI: 10.1093/nar/gkt1226. View

Kim I, Kim J, Chhetri G, Seo T . Flavobacterium humi sp. nov., a flexirubin-type pigment producing bacterium, isolated from soil. J Microbiol. 2019; 57(12):1079-1085. DOI: 10.1007/s12275-019-9350-x. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Sullivan R, Holtman M, Zylstra G, White J, Kobayashi D . Taxonomic positioning of two biological control agents for plant diseases as Lysobacter enzymogenes based on phylogenetic analysis of 16S rDNA, fatty acid composition and phenotypic characteristics. J Appl Microbiol. 2003; 94(6):1079-86. DOI: 10.1046/j.1365-2672.2003.01932.x. View

Mao Y, Song A, Li L, Yang Y, Yao Z, Wu J . A high-molecular weight exopolysaccharide from the Cs-HK1 fungus: Ultrasonic degradation, characterization and in vitro fecal fermentation. Carbohydr Polym. 2020; 246:116636. DOI: 10.1016/j.carbpol.2020.116636. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Minic Z, Marie C, Delorme C, Faurie J, Mercier G, Ehrlich D . Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase. J Bacteriol. 2006; 189(4):1351-7. PMC: 1797369. DOI: 10.1128/JB.01122-06. View

Meier-Kolthoff J, Auch A, Klenk H, Goker M . Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics. 2013; 14:60. PMC: 3665452. DOI: 10.1186/1471-2105-14-60. View

Na Y, Kim W, Kim S, Park J, Lee S, Kim S . Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens. Int Immunopharmacol. 2010; 10(3):364-70. DOI: 10.1016/j.intimp.2009.12.011. View

10.

Prajapati V, Jani G, Zala B, Khutliwala T . An insight into the emerging exopolysaccharide gellan gum as a novel polymer. Carbohydr Polym. 2013; 93(2):670-8. DOI: 10.1016/j.carbpol.2013.01.030. View

11.

Looijesteijn P, Trapet L, de Vries E, Abee T, Hugenholtz J . Physiological function of exopolysaccharides produced by Lactococcus lactis. Int J Food Microbiol. 2001; 64(1-2):71-80. DOI: 10.1016/s0168-1605(00)00437-2. View

12.

Guo Y, Pan D, Sun Y, Xin L, Li H, Zeng X . Antioxidant activity of phosphorylated exopolysaccharide produced by Lactococcus lactis subsp. lactis. Carbohydr Polym. 2013; 97(2):849-54. DOI: 10.1016/j.carbpol.2013.06.024. View

13.

Wang J, Zhao X, Tian Z, Yang Y, Yang Z . Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir. Carbohydr Polym. 2015; 125:16-25. DOI: 10.1016/j.carbpol.2015.03.003. View

14.

Yoon S, Ha S, Kwon S, Lim J, Kim Y, Seo H . Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol. 2016; 67(5):1613-1617. PMC: 5563544. DOI: 10.1099/ijsem.0.001755. View

15.

Sun J, Lu F, Luo Y, Bie L, Xu L, Wang Y . OrthoVenn3: an integrated platform for exploring and visualizing orthologous data across genomes. Nucleic Acids Res. 2023; 51(W1):W397-W403. PMC: 10320085. DOI: 10.1093/nar/gkad313. View

16.

Wall D, Nielsen U, Six J . Soil biodiversity and human health. Nature. 2015; 528(7580):69-76. DOI: 10.1038/nature15744. View

17.

Anderson A, Haywood G, DAWES E . Biosynthesis and composition of bacterial poly(hydroxyalkanoates). Int J Biol Macromol. 1990; 12(2):102-5. DOI: 10.1016/0141-8130(90)90060-n. View

18.

Dong J, Li H, Min W . Preparation, characterization and bioactivities of Athelia rolfsii exopolysaccharide-zinc complex (AEPS-zinc). Int J Biol Macromol. 2018; 113:20-28. DOI: 10.1016/j.ijbiomac.2018.01.223. View

19.

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

20.

Insulkar P, Kerkar S, Lele S . Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities. Int J Biol Macromol. 2018; 120(Pt B):1441-1450. DOI: 10.1016/j.ijbiomac.2018.09.147. View