Caspase-1 Regulates the Apoptosis and Pyroptosis Induced by Phthalocyanine Zinc-Mediated Photodynamic Therapy in Breast Cancer MCF-7 Cells

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Aug 26

PMID 37630186

Authors

Chunjie Ma

Yu Wang

Wei Chen

Ting Hou

Honglian Zhang

Hongguang Zhang

Xu Yao

Chunhui Xia

Affiliations

Soon will be listed here.

Abstract

Photodynamic therapy (PDT) is an innovative and perspective antineoplastic therapy. Tetra-α-(4-carboxyphenoxy) phthalocyanine zinc (TαPcZn)-mediated PDT (TαPcZn-PDT) has shown antitumor activity in some tumor cells, but the manner in which caspase-1 is involved in the regulation of apoptosis and pyroptosis in the TαPcZn-PDT-treated breast cancer MCF-7 cells is unclear. Therefore, effects of TαPcZn-PDT on cytotoxicity, cell viability, apoptosis, pyroptosis, cellular reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-1, caspase-3, and nuclear transcription factor-κB (NFκB) in MCF-7 cells was firstly examined in the present study. The findings demonstrated that TαPcZn-PDT resulted in the increase in cytotoxicity and the percentage of apoptotic and pyroptotic cells, the reduction in cell viability and ΔΨm, the production of ROS and the activation of caspase-1, caspase-3 and NFκB in MCF-7 cells. Furthermore, the results also revealed that siRNA-targeting caspase-1 (siRNA-caspase-1) attenuated the effect of TαPcZn-PDT on apoptosis, pyroptosis and the activation of caspase-1, caspase-3 and NFκB in MCF-7 cells. Taken together, we conclude that caspase-1 regulates the apoptosis and pyroptosis induced by TαPcZn-PDT in MCF-7 cells.

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References

Chen J, Fan T, Xie Z, Zeng Q, Xue P, Zheng T . Advances in nanomaterials for photodynamic therapy applications: Status and challenges. Biomaterials. 2020; 237:119827. DOI: 10.1016/j.biomaterials.2020.119827. View

Gholizadeh M, Doustvandi M, Mohammadnejad F, Shadbad M, Tajalli H, Brunetti O . Photodynamic Therapy with Zinc Phthalocyanine Inhibits the Stemness and Development of Colorectal Cancer: Time to Overcome the Challenging Barriers?. Molecules. 2021; 26(22). PMC: 8621355. DOI: 10.3390/molecules26226877. View

Korbelik M, Banath J, Zhang W, Gallagher P, Hode T, Lam S . N-dihydrogalactochitosan as immune and direct antitumor agent amplifying the effects of photodynamic therapy and photodynamic therapy-generated vaccines. Int Immunopharmacol. 2019; 75:105764. DOI: 10.1016/j.intimp.2019.105764. View

Lv F, Cao B, Cui Y, Liu T . Zinc phthalocyanine labelled polyethylene glycol: preparation, characterization, interaction with bovine serum albumin and near infrared fluorescence imaging in vivo. Molecules. 2012; 17(6):6348-61. PMC: 6268815. DOI: 10.3390/molecules17066348. View

Zhang H, Liu K, Xue Z, Yin H, Dong H, Jin W . High-voltage pulsed electric field plus photodynamic therapy kills breast cancer cells by triggering apoptosis. Am J Transl Res. 2018; 10(2):334-351. PMC: 5835800. View

Li N, Wang Y, Wang X, Sun N, Gong Y . Pathway network of pyroptosis and its potential inhibitors in acute kidney injury. Pharmacol Res. 2021; 175:106033. DOI: 10.1016/j.phrs.2021.106033. View

Cao H, Wang L, Yang Y, Li J, Qi Y, Li Y . An Assembled Nanocomplex for Improving both Therapeutic Efficiency and Treatment Depth in Photodynamic Therapy. Angew Chem Int Ed Engl. 2018; 57(26):7759-7763. DOI: 10.1002/anie.201802497. View

Guo W, Li Z, Huang H, Xu Z, Chen Z, Shen G . VB12-Sericin-PBLG-IR780 Nanomicelles for Programming Cell Pyroptosis via Photothermal (PTT)/Photodynamic (PDT) Effect-Induced Mitochondrial DNA (mitoDNA) Oxidative Damage. ACS Appl Mater Interfaces. 2022; 14(15):17008-17021. DOI: 10.1021/acsami.1c22804. View

Deng G, Sun Z, Li S, Peng X, Li W, Zhou L . Cell-Membrane Immunotherapy Based on Natural Killer Cell Membrane Coated Nanoparticles for the Effective Inhibition of Primary and Abscopal Tumor Growth. ACS Nano. 2018; 12(12):12096-12108. DOI: 10.1021/acsnano.8b05292. View

10.

Jiang A, Song A, Zhang C . Modes of podocyte death in diabetic kidney disease: an update. J Nephrol. 2022; 35(6):1571-1584. DOI: 10.1007/s40620-022-01269-1. View

11.

Lucky S, Soo K, Zhang Y . Nanoparticles in photodynamic therapy. Chem Rev. 2015; 115(4):1990-2042. DOI: 10.1021/cr5004198. View

12.

Zeng H, Sun M, Zhou C, Yin F, Wang Z, Hua Y . Hematoporphyrin monomethyl ether-mediated photodynamic therapy selectively kills sarcomas by inducing apoptosis. PLoS One. 2013; 8(10):e77727. PMC: 3813767. DOI: 10.1371/journal.pone.0077727. View

13.

Man S, Karki R, Kanneganti T . Molecular mechanisms and functions of pyroptosis, inflammatory caspases and inflammasomes in infectious diseases. Immunol Rev. 2017; 277(1):61-75. PMC: 5416822. DOI: 10.1111/imr.12534. View

14.

Cheng Y, Yue Y, Cao H, Geng W, Wang L, Hu X . Coassembly of hypoxia-sensitive macrocyclic amphiphiles and extracellular vesicles for targeted kidney injury imaging and therapy. J Nanobiotechnology. 2021; 19(1):451. PMC: 8714429. DOI: 10.1186/s12951-021-01192-w. View

15.

. Rilotumumab extends PFS in gastric cancer. Cancer Discov. 2014; 4(9):OF1. DOI: 10.1158/2159-8290.CD-NB2014-113. View

16.

Rajabi N, Mohammadnejad F, Doustvandi M, Shadbad M, Amini M, Tajalli H . Photodynamic therapy with zinc phthalocyanine enhances the anti-cancer effect of tamoxifen in breast cancer cell line: Promising combination treatment against triple-negative breast cancer?. Photodiagnosis Photodyn Ther. 2022; 41:103212. DOI: 10.1016/j.pdpdt.2022.103212. View

17.

Fitzmaurice C, Akinyemiju T, Al Lami F, Alam T, Alizadeh-Navaei R, Allen C . Global, Regional, and National Cancer Incidence, Mortality, Years of Life Lost, Years Lived With Disability, and Disability-Adjusted Life-Years for 29 Cancer Groups, 1990 to 2016: A Systematic Analysis for the Global Burden of Disease Study. JAMA Oncol. 2018; 4(11):1553-1568. PMC: 6248091. DOI: 10.1001/jamaoncol.2018.2706. View

18.

Doustvandi M, Mohammadnejad F, Mansoori B, Tajalli H, Mohammadi A, Mokhtarzadeh A . Photodynamic therapy using zinc phthalocyanine with low dose of diode laser combined with doxorubicin is a synergistic combination therapy for human SK-MEL-3 melanoma cells. Photodiagnosis Photodyn Ther. 2019; 28:88-97. DOI: 10.1016/j.pdpdt.2019.08.027. View

19.

Wang H, Jing G, Niu J, Yang L, Li Y, Gao Y . A mitochondria-anchored supramolecular photosensitizer as a pyroptosis inducer for potent photodynamic therapy and enhanced antitumor immunity. J Nanobiotechnology. 2022; 20(1):513. PMC: 9719646. DOI: 10.1186/s12951-022-01719-9. View

20.

de Malmanche H, Marcellin E, Reid S . Knockout of Sf-Caspase-1 generates apoptosis-resistant Sf9 cell lines: Implications for baculovirus expression. Biotechnol J. 2022; 17(7):e2100532. DOI: 10.1002/biot.202100532. View