The Effect of Aging on Nitric Oxide Production During Cerebral Ischemia and Reperfusion in Wistar Rats and Spontaneous Hypertensive Rats: An In Vivo Microdialysis Study

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Aug 26

PMID 37628930

Authors

Yasuo Ito

Harumitsu Nagoya

Masamizu Yamazato

Yoshio Asano

Masahiko Sawada

Tomokazu Shimazu

Makiko Hirayama

Toshimasa Yamamoto

Nobuo Araki

Affiliations

Soon will be listed here.

Abstract

Nitric oxide (NO) is involved in the pathogenesis of cerebral ischemic injury. Here, we investigated the effects of aging on NO production during cerebral ischemia-reperfusion (IR). Male Wister rats (WRs) were assigned to 12-month-old (older; = 5) and 3-month-old (younger; = 7) groups. Similarly, male spontaneous hypertensive rats (SHRs) were allocated to 12-month-old (older; = 6) and 3-month-old (younger; = 8) groups. After anesthesia, their NO production was monitored using in vivo microdialysis probes inserted into the left striatum and hippocampus. Forebrain cerebral IR injuries were produced via ligation of the bilateral common carotid arteries, followed by reperfusion. The change in the NO of the older rats in the SHR groups in the striatum was less compared to that of the younger rats before ischemia, during ischemia, and after reperfusion ( < 0.05). In the hippocampus, the change in the NO of the older rats in the SHR groups was lower compared to that of the younger rats after reperfusion ( < 0.05). There were no significant differences between the two WR groups. Our findings suggested that aging in SHRs affected NO production, especially in the striatum, before and during cerebral ischemia, and after reperfusion. Hypertension and aging may be important factors impacting NO production in brain IR injury.

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