Bio-Characterization and Liquid Chromatography-Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2023 Aug 26

PMID 37626952

Authors

Mani Vinoth

Sivaprakasam Sivasankari

Abdul Kareem Khaleel Ahamed

Khawla Ibrahim Alsamhary

Nouf Mohammed Al-Enazi

Neveen Abdel-Raouf

Reem Mohammed Alharbi

Rasiravathanahalli Kaveriyappan Govindarajan

Gangalla Ravi

Khaloud Mohammed Alarjani

Essam N Sholkamy

Affiliations

Soon will be listed here.

Abstract

Exopolysaccharide-producing cyanobacterial strains in biological soil crusts are described, in addition to their chemical properties and antioxidant and flocculation activities. The EPSs from Pudukkottai blackish biological soil crusts (PBBSCs) showed significant amounts of total soluble proteins (0.1687 mg/mL) and carbohydrates (0.8056 mg/mL) compared with the Ariyalur blackish biological soil crusts (ABBSCs). LC-MS analysis of the cyanobacterial polysaccharides revealed the presence of natural sugars such as ribose and glucose/mannose, and uronic acids. The FTIR spectrum showed specific peak for OH and -NH stretching, C-H stretching, and carboxylic acids as the dominant groups in EPS. The in vitro DPPH assay of EPSs from PBBSCs showed 74.3% scavenging activity. Furthermore, the reducing power was determined to be 0.59 ata 500 mg/mL concentration, respectively. The extracted EPSs from the biological soil crust flocculated Kaolin clay suspension maximum at 500 mg/mL. Consequently, the cyanobacterial strain and exopolysaccharide characterization from the sacred forest's biological soil crust were analyzed for their bioactive potential, bio-crust diversity, and distribution.

References

Castresana J . Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000; 17(4):540-52. DOI: 10.1093/oxfordjournals.molbev.a026334. View

Akaki J, Matsui Y, Kojima H, Nakajima S, Kamei K, Tamesada M . Structural analysis of monocyte activation constituents in cultured mycelia of Cordyceps sinensis. Fitoterapia. 2009; 80(3):182-7. DOI: 10.1016/j.fitote.2009.01.007. View

Herrero A, Muro-Pastor A, Flores E . Nitrogen control in cyanobacteria. J Bacteriol. 2001; 183(2):411-25. PMC: 94895. DOI: 10.1128/JB.183.2.411-425.2001. View

Khattar J, Singh D, Jindal N, Kaur N, Singh Y, Rahi P . Isolation and characterization of exopolysaccharides produced by the cyanobacterium Limnothrix redekei PUPCCC 116. Appl Biochem Biotechnol. 2010; 162(5):1327-38. DOI: 10.1007/s12010-010-8922-3. View

Roncero-Ramos B, Munoz-Martin M, Chamizo S, Fernandez-Valbuena L, Mendoza D, Perona E . Polyphasic evaluation of key cyanobacteria in biocrusts from the most arid region in Europe. PeerJ. 2019; 7:e6169. PMC: 6321753. DOI: 10.7717/peerj.6169. View

Valentao P, Fernandes E, Carvalho F, Andrade P, Seabra R, Bastos M . Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. J Agric Food Chem. 2002; 50(17):4989-93. DOI: 10.1021/jf020225o. View

Xu H, Raanan H, Dai G, Oren N, Berkowicz S, Murik O . Reading and surviving the harsh conditions in desert biological soil crust: the cyanobacterial viewpoint. FEMS Microbiol Rev. 2021; 45(6). DOI: 10.1093/femsre/fuab036. View

Vinoth M, Sivasankari S, Ahamed A, Al-Arjani A, Abd Allah E, Baskar K . Biological soil crust (BSC) is an effective biofertilizer on (L.). Saudi J Biol Sci. 2020; 27(9):2325-2332. PMC: 7451670. DOI: 10.1016/j.sjbs.2020.04.022. View

Hou W, Chen Y, Chen H, Lin Y, Yang L, Lee M . Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57). J Agric Food Chem. 2001; 49(6):2978-81. DOI: 10.1021/jf0100705. View

10.

Costa O, Raaijmakers J, Kuramae E . Microbial Extracellular Polymeric Substances: Ecological Function and Impact on Soil Aggregation. Front Microbiol. 2018; 9:1636. PMC: 6064872. DOI: 10.3389/fmicb.2018.01636. View

11.

Govindarajan R, Khanongnuch C, Mathivanan K, Shyu D, Sharma K, De Mandal S . biotransformation of tea using tannase produced by 41. J Food Sci Technol. 2021; 58(8):3235-3242. PMC: 8249508. DOI: 10.1007/s13197-021-05018-3. View

12.

Li S, Huang R, Shah N, Tao X, Xiong Y, Wei H . Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315. J Dairy Sci. 2014; 97(12):7334-43. DOI: 10.3168/jds.2014-7912. View

13.

Shah V, Ray A, Garg N, Madamwar D . Characterization of the extracellular polysaccharide produced by a marine cyanobacterium, Cyanothece sp. ATCC 51142, and its exploitation toward metal removal from solutions. Curr Microbiol. 2000; 40(4):274-8. DOI: 10.1007/s002849910054. View

14.

Hossain M, Ratnayake R, Meerajini K, Wasantha Kumara K . Antioxidant properties in some selected cyanobacteria isolated from fresh water bodies of Sri Lanka. Food Sci Nutr. 2016; 4(5):753-8. PMC: 5011383. DOI: 10.1002/fsn3.340. View

15.

Ismail B, Nampoothiri K . Production, purification and structural characterization of an exopolysaccharide produced by a probiotic Lactobacillus plantarum MTCC 9510. Arch Microbiol. 2010; 192(12):1049-57. DOI: 10.1007/s00203-010-0636-y. View

16.

Itoh H, Noda H, Amano H, Zhuaug C, Mizuno T, Ito H . Antitumor activity and immunological properties of marine algal polysaccharides, especially fucoidan, prepared from Sargassum thunbergii of Phaeophyceae. Anticancer Res. 1993; 13(6A):2045-52. View

17.

Benzie I, Strain J . Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999; 299:15-27. DOI: 10.1016/s0076-6879(99)99005-5. View

18.

Delattre C, Pierre G, Laroche C, Michaud P . Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides. Biotechnol Adv. 2016; 34(7):1159-1179. DOI: 10.1016/j.biotechadv.2016.08.001. View

19.

Redmile-Gordon M, Evershed R, Hirsch P, White R, Goulding K . Soil organic matter and the extracellular microbial matrix show contrasting responses to C and N availability. Soil Biol Biochem. 2015; 88:257-267. PMC: 4534311. DOI: 10.1016/j.soilbio.2015.05.025. View

20.

Moreira-Grez B, Tam K, Cross A, Yong J, Kumaresan D, Nevill P . The Bacterial Microbiome Associated With Arid Biocrusts and the Biogeochemical Influence of Biocrusts Upon the Underlying Soil. Front Microbiol. 2019; 10:2143. PMC: 6768011. DOI: 10.3389/fmicb.2019.02143. View