Efficacy and Mechanism Study of 6S-5-Methyltetrahydrofolate-Calcium Against Telencephalon Infarction Injury in Zebrafish Model of Ischemic Stroke

Overview

Journal Mol Neurobiol

Specialties Molecular Biology
Neurology

Date 2023 Aug 25

PMID 37624487

Authors

Xin-Ni Bin

Tao-Jun Xu

Han-Peng Zou

Zenglin Lian

Yongzhi Cheng

Jia-Qi Wu

Ming-Fang He

Affiliations

Soon will be listed here.

Abstract

Ischemic stroke is a heterogeneous brain injury with complex pathophysiology and it is also a time sensitive neurological injury disease. At present, the treatment options for ischemic stroke are still limited. 6S-5-methyltetrahydrofolate-calcium (MTHF-Ca) is the calcium salt of the predominant form of dietary folate in circulation. MTHF-Ca has potential neuroprotective effect on neurocytes, but whether it can be used for ischemic stroke treatment remains unknown. We established zebrafish ischemic stroke model through photothrombotic method to evaluate the protective effect of MTHF-Ca on the ischemic brain injury of zebrafish. We demonstrated that MTHF-Ca reduced the brain damage by reducing motor dysfunction and neurobehavioral defects of zebrafish with telencephalon infarction injury. MTHF-Ca counteracted oxidative damages after Tel injury by increasing the activities of GSH-Px and SOD and decreasing the content of MDA. RNA-seq and RT-qPCR results showed that MTHF-Ca played a neuroprotective role by alleviating neuroinflammation, inhibiting blood coagulation, and neuronal apoptosis processes. Overall, we have demonstrated that MTHF-Ca has neuroprotective effect in ischemic stroke and can be used as a potential treatment for ischemic stroke.

Citing Articles

Drug screening for ischemic stroke using larvae and adult zebrafish model: a review.

Nayaka N, Adnyana I, Anggadiredja K, Wibowo I Lab Anim Res. 2025; 41(1):1.

PMID: 39743611 PMC: 11694438. DOI: 10.1186/s42826-024-00232-4.

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