Okadaic Acid Detection Through a Rapid and Sensitive Amplified Luminescent Proximity Homogeneous Assay

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2023 Aug 25

PMID 37624258

Authors

Yuan Qin

Jiayu Li

Jiani Kuang

Sicheng Shen

Xiumei Zhou

Xueqin Zhao

Biao Huang

Bingnan Han

Affiliations

Soon will be listed here.

Abstract

Okadaic acid (OA), a marine biotoxin produced by microalgae, poses a significant threat to mariculture, seafood safety, and human health. The establishment of a novel, highly sensitive detection method for OA would have significant practical and scientific implications. Therefore, the purpose of this study was to develop an innovative approach for OA detection. A competitive amplified luminescent proximity homogeneous assay (AlphaLISA) was developed using the principle of specific antigen-antibody binding based on the energy transfer between chemiluminescent microspheres. The method was non-washable, sensitive, and rapid, which could detect 2 × 10-200 ng/mL of OA within 15 min, and the detection limit was 4.55 × 10 ng/mL. The average intra- and inter-assay coefficients of variation were 2.54% and 6.26%, respectively. Detection of the actual sample results exhibited a good correlation with high-performance liquid chromatography. In conclusion, a simple, rapid, sensitive, and accurate AlphaLISA method was established for detecting OA and is expected to significantly contribute to marine biotoxin research.

Citing Articles

Development of a Quick and Highly Sensitive Amplified Luminescent Proximity Homogeneous Assay for Detection of Saxitoxin in Shellfish.

Zhao C, Zhang Z, Li J, Lu Y, Ma F, Wang Z Toxins (Basel). 2024; 16(8).

PMID: 39195751 PMC: 11360761. DOI: 10.3390/toxins16080341.

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