The Adenylate Cyclase-Encoding Gene Is Involved in Mycoparasitism

Overview

Journal J Fungi (Basel)

Date 2023 Aug 25

PMID 37623632

Authors

Shu-Fan Yu

Zhan-Bin Sun

Shi-Dong Li

Ya-Feng Hu

Qing Ren

Jia-Liang Xu

Han-Jian Song

Man-Hong Sun

Affiliations

Soon will be listed here.

Abstract

is an excellent biocontrol fungus against numerous fungal plant pathogens. The cAMP signaling pathway is a crucial signal transduction pathway in fungi. To date, the role of the cAMP signaling pathway in mycoparasitism remains unknown. An adenylate cyclase-encoding gene, (an important component of the cAMP signaling pathway), was previously screened from 67-1, and its expression level was dramatically upregulated during the mycoparasitization of the sclerotia of . In this study, the function of in mycoparasitism was explored through gene knockout and complementation. The obtained results show that the deletion of influenced the growth rate and colony morphology of , as well as the tolerance to NaCl and HO stress. The mycoparasitic effects on the sclerotia of and the biocontrol capacity on soybean Sclerotinia stem rot in ∆ and ∆ were both attenuated compared with that of the wild-type strain and complementation transformants. To understand the regulatory mechanism of during mycoparasitism, transcriptomic analysis was conducted between the wild-type strain and knockout mutant. A number of biocontrol-related genes, including genes encoding cell wall-degrading enzymes and transporters, were significantly differentially expressed during mycoparasitism, suggesting that may be involved in mycoparasitism by regulating the expression of these DEGs. These findings provide insight for further exploring the molecular mechanism of mycoparasitism.

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