Multi-Walled Carbon Nanotube Array Modified Electrode with 3D Sensing Interface As Electrochemical DNA Biosensor for Multidrug-Resistant Gene Detection

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 Aug 25

PMID 37622850

Authors

Ruiting Chen

Hejing Chen

Huaping Peng

Yanjie Zheng

Zhen Lin

Xinhua Lin

Affiliations

Soon will be listed here.

Abstract

Drug resistance in cancer is associated with overexpression of the multidrug resistance (MDR1) gene, leading to the failure of cancer chemotherapy treatment. Therefore, the establishment of an effective method for the detection of the MDR1 gene is extremely crucial in cancer clinical therapy. Here, we report a novel DNA biosensor based on an aligned multi-walled carbon nanotube (MWCNT) array modified electrode with 3D nanostructure for the determination of the MDR1 gene. The microstructure of the modified electrode was observed by an atomic force microscope (AFM), which demonstrated that the electrode interface was arranged in orderly needle-shaped protrusion arrays. The electrochemical properties of the biosensor were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Chronocoulometry (CC) was used for the quantitative detection of the MDR1 gene. Taking advantage of the good conductivity and large electrode area of the MWCNT arrays, this electrochemical DNA sensor achieved a dynamic range from 1.0 × 10 M to 1.0 × 10 M with a minimal detection limit of 6.4 × 10 M. In addition, this proposed DNA biosensor exhibited high sensitivity, selectivity, and stability, which may be useful for the trace analysis of the MDR1 gene in complex samples.

Citing Articles

Multi-walled carbon nanotubes functionalized with a new Schiff base containing phenylboronic acid residues: application to the development of a bienzymatic glucose biosensor using a response surface methodology approach.

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