Engineering Co-utilization of Glucose and Xylose for Chemical Overproduction from Lignocellulose

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2023 Aug 24

PMID 37620399

Authors

Jiaoqi Gao

Wei Yu

Yunxia Li

Mingjie Jin

Lun Yao

Yongjin J Zhou

Affiliations

Soon will be listed here.

Abstract

Bio-refining lignocellulose could provide a sustainable supply of fuels and fine chemicals; however, the challenges associated with the co-utilization of xylose and glucose typically compromise the efficiency of lignocellulose conversion. Here we engineered the industrial yeast Ogataea polymorpha (Hansenula polymorpha) for lignocellulose biorefinery by facilitating the co-utilization of glucose and xylose to optimize the production of free fatty acids (FFAs) and 3-hydroxypropionic acid (3-HP) from lignocellulose. We rewired the central metabolism for the enhanced supply of acetyl-coenzyme A and nicotinamide adenine dinucleotide phosphate hydrogen, obtaining 30.0 g l of FFAs from glucose, with productivity of up to 0.27 g l h. Strengthening xylose uptake and catabolism promoted the synchronous utilization of glucose and xylose, which enabled the production of 38.2 g l and 7.0 g l FFAs from the glucose-xylose mixture and lignocellulosic hydrolysates, respectively. Finally, this efficient cell factory was metabolically transformed for 3-HP production with the highest titer of 79.6 g l in fed-batch fermentation in mixed glucose and xylose.

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