Transcriptome Analysis of Hydrogen Inhibits Osteoclastogenesis of Mouse Bone Marrow Mononuclear Cells

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2023 Aug 24

PMID 37614423

Authors

Yong Liu

Wei Wang

Yong Zeng

Hui Zeng

Affiliations

Soon will be listed here.

Abstract

Hydrogen (H) is a major biodegradation product of implanted magnesium (Mg) alloys that are commonly used in the healing of bone fractures. Our earlier study showed that H can inhibit mouse bone marrow mononuclear cell (BMMC) osteoclastogenesis during the differentiation of these cells into osteoclasts, thereby facilitating fracture healing. However, the way by which H inhibits osteoclastogenesis remains to be elucidated. The present study used RNA-sequencing to study the transcriptome of H-exposed BMMCs in an osteoclast-induced environment and identified the target genes and signaling pathways through which H exerts its biological effects. Several upregulated genes were identified: , , , , , , , , and . Several downregulated genes were also revealed: , , , , , , , , and 5. These differentially expressed genes were mainly involved in osteoclast differentiation cascades, as well as PI3K-AKT, Forkhead box O (FoxO), MAPK, peroxisome proliferator-activated receptor (PPAR), TNF, TGF-β, JAK-STAT, RAS, VEGF, hypoxia-inducible factor (HIF-1) and AMPK signaling pathways. In summary, the present study revealed the key genes and signaling pathways involved in the H-mediated inhibition of osteoclastogenesis, thereby providing a theoretical basis for the significance of H and an experimental basis for the application of Mg alloys in the treatment of osteoporosis.

Citing Articles

Bioinformatics Analysis and Experimental Validation of Differential Genes and Pathways in Bone Nonunions.

Xiong W, Shu X, Huang L, He S, Liu L, Li S Biochem Genet. 2024; 62(6):4494-4517.

PMID: 38324134 DOI: 10.1007/s10528-023-10633-0.

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