The Influence of Risk Factors on Bovine Leukemia Virus Infection and Proviral Load in Egyptian Cattle

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2023 Aug 23

PMID 37610507

Authors

Rania Hamada

Ragab M Fereig

Samy Metwally

Affiliations

Soon will be listed here.

Abstract

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leucosis (EBL), which affects cattle globally. In Egypt, BLV control strategies have been ignored because of the shortage of BLV research studies and the silent infection in most animals. This study aimed to identify the risk factors associated with the prevalence of BLV among dairy and beef cattle from six different geographic and climatic provinces in Egypt. Additionally, risk factors affecting the BLV proviral load (PVL) among the positive cattle were targeted. The total BLV prevalence in cattle from six investigated Egyptian provinces was 24.2% (105/433), while the mean PVL (8651.6 copies /10 white blood cells) was absolutely high as estimated by the BLV-CoCoMo-quantitative polymerase chain reaction (qPCR)-2 assay. Analysis of the influence of risk factors (age, sex, breed, production type, farm size, and location) on BLV prevalence indicated that the Holstein breed (OR = 1.582, p = 0.007), beef cattle (OR = 1.088, p = 0.0001), large-size farms (OR = 1.26, p = 0.0001), and cattle from Damietta (OR = 1.43, p = 0.0001) and Cairo (OR = 1.16, p = 0.0001) were ultimately proven the most important risks for BLV infection. The risk factors were analyzed considering the BLV PVL levels in the BLV-positive cases. Significantly high PVL (HPVL) levels were observed in cattle > 5 years old (p < 0.0001), females (p = 0.0008), Holstein (p < 0.0001), dairy cows (p = 0.0053), large-size farms (p < 0.0001), and cattle from Damietta (p < 0.0001) compared to other categories. Contrary, no significant differences in PVL levels were reported between the Native and Mixed cattle breeds (p = 0.13). Ultimately, the logistic regression model indicated that the probability of carrying HPVL in cattle > 5 years is 1.27 (95% CI: 1.03-2.09, p < 0.001) times more likely compared to cattle < 2 years old. In conclusion, the findings were valuably correlating the BLV prevalence with PVL as an indicator of the risk of BLV infection.

References

Bartlett P, Norby B, Byrem T, Parmelee A, Ledergerber J, Erskine R . Bovine leukemia virus and cow longevity in Michigan dairy herds. J Dairy Sci. 2013; 96(3):1591-7. DOI: 10.3168/jds.2012-5930. View

Bartlett P, Ruggiero V, Hutchinson H, Droscha C, Norby B, Sporer K . Current Developments in the Epidemiology and Control of Enzootic Bovine Leukosis as Caused by Bovine Leukemia Virus. Pathogens. 2020; 9(12). PMC: 7766781. DOI: 10.3390/pathogens9121058. View

Baumgartener L, Olson C, Miller J, Van Der Maaten M . Survey for antibodies to leukemia (C-type) virus in cattle. J Am Vet Med Assoc. 1975; 166(3):249-51. View

Frie M, Coussens P . Bovine leukemia virus: a major silent threat to proper immune responses in cattle. Vet Immunol Immunopathol. 2015; 163(3-4):103-14. DOI: 10.1016/j.vetimm.2014.11.014. View

Gutierrez G, Rodriguez S, de Brogniez A, Gillet N, Golime R, Burny A . Vaccination against δ-retroviruses: the bovine leukemia virus paradigm. Viruses. 2014; 6(6):2416-27. PMC: 4074934. DOI: 10.3390/v6062416. View

Hamada R, Metwally S, Polat M, Borjigin L, Ali A, Abdel-Hady A . Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle. Front Vet Sci. 2020; 7:608. PMC: 7511665. DOI: 10.3389/fvets.2020.00608. View

Hopkins S, Digiacomo R . Natural transmission of bovine leukemia virus in dairy and beef cattle. Vet Clin North Am Food Anim Pract. 1997; 13(1):107-28. DOI: 10.1016/s0749-0720(15)30367-4. View

Jimba M, Takeshima S, Matoba K, Endoh D, Aida Y . BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm. Retrovirology. 2010; 7:91. PMC: 2988707. DOI: 10.1186/1742-4690-7-91. View

Juliarena M, Barrios C, Ceriani M, Esteban E . Hot topic: Bovine leukemia virus (BLV)-infected cows with low proviral load are not a source of infection for BLV-free cattle. J Dairy Sci. 2016; 99(6):4586-4589. DOI: 10.3168/jds.2015-10480. View

10.

Kettmann R, Cortadas J, Cuny G, Ghysdael J, MAMMERICKX M, Burny A . Integration of bovine leukemia virus DNA in the bovine genome. Proc Natl Acad Sci U S A. 1979; 76(10):4822-6. PMC: 413029. DOI: 10.1073/pnas.76.10.4822. View

11.

Kobayashi S, Tsutsui T, Yamamoto T, Hayama Y, Kameyama K, Konishi M . Risk factors associated with within-herd transmission of bovine leukemia virus on dairy farms in Japan. BMC Vet Res. 2010; 6:1. PMC: 2835688. DOI: 10.1186/1746-6148-6-1. View

12.

Kobayashi S, Hidano A, Tsutsui T, Yamamoto T, Hayama Y, Nishida T . Analysis of risk factors associated with bovine leukemia virus seropositivity within dairy and beef breeding farms in Japan: a nationwide survey. Res Vet Sci. 2013; 96(1):47-53. DOI: 10.1016/j.rvsc.2013.11.014. View

13.

Lo C, Borjigin L, Saito S, Fukunaga K, Saitou E, Okazaki K . BoLA-DRB3 Polymorphism is Associated with Differential Susceptibility to Bovine Leukemia Virus-Induced Lymphoma and Proviral Load. Viruses. 2020; 12(3). PMC: 7150773. DOI: 10.3390/v12030352. View

14.

Kyaw Moe K, Polat M, Borjigin L, Matsuura R, Hein S, Moe H . New evidence of bovine leukemia virus circulating in Myanmar cattle through epidemiological and molecular characterization. PLoS One. 2020; 15(2):e0229126. PMC: 7034883. DOI: 10.1371/journal.pone.0229126. View

15.

More S, Bicout D, Botner A, Butterworth A, Calistri P, Depner K . Low atmospheric pressure system for stunning broiler chickens. EFSA J. 2020; 15(12):e05056. PMC: 7010056. DOI: 10.2903/j.efsa.2017.5056. View

16.

Nagy D, Tyler J, Kleiboeker S . Decreased periparturient transmission of bovine leukosis virus in colostrum-fed calves. J Vet Intern Med. 2007; 21(5):1104-7. DOI: 10.1892/0891-6640(2007)21[1104:dptobl]2.0.co;2. View

17.

Nakatsuchi A, Watanuki S, Borjigin L, Sato H, Bai L, Matsuura R . Polymorphism Controls Proviral Load and Infectivity of Bovine Leukemia Virus (BLV) in Milk. Pathogens. 2022; 11(2). PMC: 8879029. DOI: 10.3390/pathogens11020210. View

18.

Norby B, Bartlett P, Byrem T, Erskine R . Effect of infection with bovine leukemia virus on milk production in Michigan dairy cows. J Dairy Sci. 2016; 99(3):2043-2052. DOI: 10.3168/jds.2015-10089. View

19.

Ohno A, Takeshima S, Matsumoto Y, Aida Y . Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014. Virus Res. 2015; 210:283-90. DOI: 10.1016/j.virusres.2015.08.020. View

20.

Sato H, Watanuki S, Murakami H, Sato R, Ishizaki H, Aida Y . Development of a luminescence syncytium induction assay (LuSIA) for easily detecting and quantitatively measuring bovine leukemia virus infection. Arch Virol. 2018; 163(6):1519-1530. DOI: 10.1007/s00705-018-3744-7. View