Evaluation of Bioremediation Potentiality of Bacillus Mojavensis Isolated from Wastewater for the Elimination of Reactive Yellow 145 and Methyl Orange

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2023 Aug 23

PMID 37610439

Authors

Rajaa Sennaj

Khadija Dari

Mohammed Timinouni

Taoufiq Benali

Aziz Fassouane

Kaoutar Harboul

Hinde Aassila

Affiliations

Soon will be listed here.

Abstract

Textile industry waste has become one of the largest polluters in the world. In recent years, there has been a growing awareness of the need for sustainable and eco-friendly practices for the treatment of dye-laden effluents. Overall, this study highlights the potential of bioremediation as a sustainable solution for wastewater treatment. The Bacillus mojavensis isolated from wastewater and identified using 16S rRNA degraded reactive yellow 145 and methyl orange in 36 h of incubation, this decolorization was affected by pH, temperature, dye concentration, glucose concentration, source of nitrogen, type of dye, and agitation. Our study found that the optimal conditions for total decolorization of dyes were achieved by incubating B. mojavensis at 46 °C, pH 9, with 1 g/L of glucose and 2 g/L of peptone. The azoreductase activity, FT-IR analysis, and UV-visible spectrum before and after total decolorization indicated that it was a dye degradation rather than biosorption in surface Celle. In addition, the study of phytotoxicity show the metabolites of degradation are not phytotoxic in Lens esculenta seeds. In conclusion, our results suggest the use of this bacterium as an environmentally friendly and also cost-effective method, making it an attractive option for industries looking to reduce their environmental impact.

References

Al-Tohamy R, Ali S, Li F, Okasha K, Mahmoud Y, Elsamahy T . A critical review on the treatment of dye-containing wastewater: Ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety. Ecotoxicol Environ Saf. 2022; 231:113160. DOI: 10.1016/j.ecoenv.2021.113160. View

Holkar C, Jadhav A, Pinjari D, Mahamuni N, Pandit A . A critical review on textile wastewater treatments: Possible approaches. J Environ Manage. 2016; 182:351-366. DOI: 10.1016/j.jenvman.2016.07.090. View

Khan S, Malik A . Degradation of Reactive Black 5 dye by a newly isolated bacterium Pseudomonas entomophila BS1. Can J Microbiol. 2016; 62(3):220-32. DOI: 10.1139/cjm-2015-0552. View

Al-Ansari M, Li Z, Masood A, Rajaselvam J . Decolourization of azo dye using a batch bioreactor by an indigenous bacterium Enterobacter aerogenes ES014 from the waste water dye effluent and toxicity analysis. Environ Res. 2021; 205:112189. DOI: 10.1016/j.envres.2021.112189. View

Ameenudeen S, Unnikrishnan S, Ramalingam K . Statistical optimization for the efficacious degradation of reactive azo dyes using Acinetobacter baumannii JC359. J Environ Manage. 2020; 279:111512. DOI: 10.1016/j.jenvman.2020.111512. View

Garg N, Garg A, Mukherji S . Eco-friendly decolorization and degradation of reactive yellow 145 textile dye by Pseudomonas aeruginosa and Thiosphaera pantotropha. J Environ Manage. 2020; 263:110383. DOI: 10.1016/j.jenvman.2020.110383. View

Sennaj R, Lemriss S, Souiri A, Kabbaj S, Chafik A, Essamadi A . Eco-friendly degradation of reactive red 195, reactive blue 214, and reactive yellow 145 by Klebsiella pneumoniae MW815592 isolated from textile waste. J Microbiol Methods. 2022; 204:106659. DOI: 10.1016/j.mimet.2022.106659. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Felsenstein J . CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution. 2017; 39(4):783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x. View

10.

Moutaouakkil A, Zeroual Y, Dzayri F, Talbi M, Lee K, Blaghen M . Purification and partial characterization of azoreductase from Enterobacter agglomerans. Arch Biochem Biophys. 2003; 413(1):139-46. DOI: 10.1016/s0003-9861(03)00096-1. View

11.

Zimmermann T, Kulla H, Leisinger T . Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. Eur J Biochem. 1982; 129(1):197-203. DOI: 10.1111/j.1432-1033.1982.tb07040.x. View

12.

Moutaouakkil A, Zeroual Y, Dzayri F, Talbi M, Lee K, Blaghen M . Decolorization of azo dyes with Enterobacter agglomerans immobilized in different supports by using fluidized bed bioreactor. Curr Microbiol. 2004; 48(2):124-9. DOI: 10.1007/s00284-003-4143-0. View

13.

Elizalde-Gonzalez M, Fuentes-Ramirez L, Guevara-Villa M . Degradation of immobilized azo dyes by Klebsiella sp. UAP-b5 isolated from maize bioadsorbent. J Hazard Mater. 2008; 161(2-3):769-74. DOI: 10.1016/j.jhazmat.2008.04.023. View

14.

Barathi S, ArulJothi K, Karthik C, Padikasan I, Ashokkumar V . Biofilm mediated decolorization and degradation of reactive red 170 dye by the bacterial consortium isolated from the dyeing industry wastewater sediments. Chemosphere. 2021; 286(Pt 3):131914. DOI: 10.1016/j.chemosphere.2021.131914. View

15.

Chen K, Wu J, Hwang S . Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol. 2003; 101(1):57-68. DOI: 10.1016/s0168-1656(02)00303-6. View

16.

Salony , Mishra S, Bisaria V . Production and characterization of laccase from Cyathus bulleri and its use in decolourization of recalcitrant textile dyes. Appl Microbiol Biotechnol. 2005; 71(5):646-53. DOI: 10.1007/s00253-005-0206-4. View

17.

Singh A, Chaudhary S, Kumar S, Kumar A, Singh A, Yadav A . Biodegradation of Reactive Yellow-145 azo dye using bacterial consortium: A deterministic analysis based on degradable Metabolite, phytotoxicity and genotoxicity study. Chemosphere. 2022; 300:134504. DOI: 10.1016/j.chemosphere.2022.134504. View