Reoccurring Escherichia Coli O157:H7 Strain Linked to Leafy Greens-Associated Outbreaks, 2016-2019

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2023 Aug 23

PMID 37610207

Authors

Jessica C Chen

Kane Patel

Peyton A Smith

Eshaw Vidyaprakash

Caroline Snyder

Kaitlin A Tagg

Hattie E Webb

Morgan N Schroeder

Lee S Katz

Lori A Rowe

Dakota Howard

Taylor Griswold

Rebecca L Lindsey

Heather A Carleton

Affiliations

Soon will be listed here.

Abstract

Genomic characterization of an Escherichia coli O157:H7 strain linked to leafy greens-associated outbreaks dates its emergence to late 2015. One clade has notable accessory genomic content and a previously described mutation putatively associated with increased arsenic tolerance. This strain is a reoccurring, emerging, or persistent strain causing illness over an extended period.

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References

Punshon T, Jackson B, Meharg A, Warczack T, Scheckel K, Guerinot M . Understanding arsenic dynamics in agronomic systems to predict and prevent uptake by crop plants. Sci Total Environ. 2017; 581-582:209-220. PMC: 5303541. DOI: 10.1016/j.scitotenv.2016.12.111. View

Mead P, Griffin P . Escherichia coli O157:H7. Lancet. 1998; 352(9135):1207-12. DOI: 10.1016/S0140-6736(98)01267-7. View

Marshall K, Hexemer A, Seelman S, Fatica M, Blessington T, Hajmeer M . Lessons Learned from a Decade of Investigations of Shiga Toxin-Producing Escherichia coli Outbreaks Linked to Leafy Greens, United States and Canada. Emerg Infect Dis. 2020; 26(10):2319-2328. PMC: 7510726. DOI: 10.3201/eid2610.191418. View

Redondo-Salvo S, Bartomeus-Penalver R, Vielva L, Tagg K, Webb H, Fernandez-Lopez R . COPLA, a taxonomic classifier of plasmids. BMC Bioinformatics. 2021; 22(1):390. PMC: 8325299. DOI: 10.1186/s12859-021-04299-x. View

Tolar B, Joseph L, Schroeder M, Stroika S, Ribot E, Hise K . An Overview of PulseNet USA Databases. Foodborne Pathog Dis. 2019; 16(7):457-462. PMC: 6653802. DOI: 10.1089/fpd.2019.2637. View

Seemann T . Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014; 30(14):2068-9. DOI: 10.1093/bioinformatics/btu153. View

Bouckaert R, Vaughan T, Barido-Sottani J, Duchene S, Fourment M, Gavryushkina A . BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis. PLoS Comput Biol. 2019; 15(4):e1006650. PMC: 6472827. DOI: 10.1371/journal.pcbi.1006650. View

Page A, Cummins C, Hunt M, Wong V, Reuter S, Holden M . Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics. 2015; 31(22):3691-3. PMC: 4817141. DOI: 10.1093/bioinformatics/btv421. View

Brynildsrud O, Bohlin J, Scheffer L, Eldholm V . Rapid scoring of genes in microbial pan-genome-wide association studies with Scoary. Genome Biol. 2016; 17(1):238. PMC: 5124306. DOI: 10.1186/s13059-016-1108-8. View

10.

Bielaszewska M, Schmidt H, Liesegang A, Prager R, Rabsch W, Tschape H . Cattle can be a reservoir of sorbitol-fermenting shiga toxin-producing Escherichia coli O157:H(-) strains and a source of human diseases. J Clin Microbiol. 2000; 38(9):3470-3. PMC: 87410. DOI: 10.1128/JCM.38.9.3470-3473.2000. View

11.

Cherry J . Recent Genetic Changes Affecting Enterohemorrhagic Escherichia coli Causing Recurrent Outbreaks. Microbiol Spectr. 2022; 10(3):e0050122. PMC: 9241674. DOI: 10.1128/spectrum.00501-22. View

12.

Katz L, Griswold T, Williams-Newkirk A, Wagner D, Petkau A, Sieffert C . A Comparative Analysis of the Lyve-SET Phylogenomics Pipeline for Genomic Epidemiology of Foodborne Pathogens. Front Microbiol. 2017; 8:375. PMC: 5346554. DOI: 10.3389/fmicb.2017.00375. View

13.

Scallan E, Hoekstra R, Angulo F, Tauxe R, Widdowson M, Roy S . Foodborne illness acquired in the United States--major pathogens. Emerg Infect Dis. 2011; 17(1):7-15. PMC: 3375761. DOI: 10.3201/eid1701.p11101. View

14.

Lin Y, Yuan J, Kolmogorov M, Shen M, Chaisson M, Pevzner P . Assembly of long error-prone reads using de Bruijn graphs. Proc Natl Acad Sci U S A. 2016; 113(52):E8396-E8405. PMC: 5206522. DOI: 10.1073/pnas.1604560113. View

15.

Heiman K, Mody R, Johnson S, Griffin P, Gould L . Escherichia coli O157 Outbreaks in the United States, 2003-2012. Emerg Infect Dis. 2015; 21(8):1293-1301. PMC: 4517704. DOI: 10.3201/eid2108.141364. View