Feasibility Insights into the Application of E1 in Animal Feed to Eliminate Non-starch Polysaccharides

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Aug 23

PMID 37608941

Authors

Gen Li

Yue Yuan

Bowen Jin

Zhiqiang Zhang

Bilal Murtaza

Hong Zhao

Xiaoyu Li

Lili Wang

Yongping Xu

Affiliations

Soon will be listed here.

Abstract

The goal of the research was to find alternative protein sources for animal farming that are efficient and cost-effective. The researchers focused on distillers dried grains with solubles (DDGS), a co-product of bioethanol production that is rich in protein but limited in its use as a feed ingredient due to its high non-starch polysaccharides (NSPs) content, particularly for monogastric animals. The analysis of the E1 genome revealed the presence of 372 genes related to Carbohydrate-Active enzymes (CAZymes), with 98 of them associated with NSPs degrading enzymes that target cellulose, hemicellulose, and pectin. Additionally, although lignin is not an NSP, two lignin-degrading enzymes were also examined because the presence of lignin alongside NSPs can hinder the catalytic effect of enzymes on NSPs. To confirm the catalytic ability of the degrading enzymes, an enzyme activity assay was conducted. The results demonstrated that the endoglucanase activity reached 5.37 U/mL, while beta-glucosidase activity was 4.60 U/mL. The filter paper experiments did not detect any reducing sugars. The xylanase and beta-xylosidase activities were measured at 11.05 and 4.16 U/mL, respectively. Furthermore, the pectate lyase and pectin lyase activities were found to be 8.19 and 2.43 U/mL, respectively. The activities of laccase and MnP were determined as 1.87 and 4.30 U/mL, respectively. The researchers also investigated the effect of E1 on the degradation of NSPs through the solid-state fermentation of DDGS. After 240 h of fermentation, the results showed degradation rates of 11.86% for hemicellulose, 11.53% for cellulose, and 8.78% for lignin. Moreover, the crude protein (CP) content of DDGS increased from 26.59% to 30.59%. In conclusion, this study demonstrated that E1 possesses various potential NSPs degrading enzymes that can effectively eliminate NSPs in feed. This process improves the quality and availability of the feed, which is important for animal farming as it seeks alternative protein sources to replace traditional nutrients.

References

Baramee S, Teeravivattanakit T, Phitsuwan P, Waeonukul R, Pason P, Tachaapaikoon C . A novel GH6 cellobiohydrolase from Paenibacillus curdlanolyticus B-6 and its synergistic action on cellulose degradation. Appl Microbiol Biotechnol. 2016; 101(3):1175-1188. DOI: 10.1007/s00253-016-7895-8. View

Lopez-Mondejar R, Zuhlke D, Vetrovsky T, Becher D, Riedel K, Baldrian P . Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199. Biotechnol Biofuels. 2016; 9:104. PMC: 4867992. DOI: 10.1186/s13068-016-0518-x. View

Borin G, Sanchez C, de Souza A, de Santana E, de Souza A, Leme A . Comparative Secretome Analysis of Trichoderma reesei and Aspergillus niger during Growth on Sugarcane Biomass. PLoS One. 2015; 10(6):e0129275. PMC: 4460134. DOI: 10.1371/journal.pone.0129275. View

Koutaniemi S, Tenkanen M . Action of three GH51 and one GH54 α-arabinofuranosidases on internally and terminally located arabinofuranosyl branches. J Biotechnol. 2016; 229:22-30. DOI: 10.1016/j.jbiotec.2016.04.050. View

Canas A, Camarero S . Laccases and their natural mediators: biotechnological tools for sustainable eco-friendly processes. Biotechnol Adv. 2010; 28(6):694-705. DOI: 10.1016/j.biotechadv.2010.05.002. View

Wang H, Pu Y, Ragauskas A, Yang B . From lignin to valuable products-strategies, challenges, and prospects. Bioresour Technol. 2018; 271:449-461. DOI: 10.1016/j.biortech.2018.09.072. View

Terefe Z, Omwamba M, Nduko J . Effect of solid state fermentation on proximate composition, antinutritional factors and in vitro protein digestibility of maize flour. Food Sci Nutr. 2021; 9(11):6343-6352. PMC: 8565243. DOI: 10.1002/fsn3.2599. View

Ghio S, Bradanini M, Garrido M, Ontanon O, Piccinni F, Marrero Diaz de Villegas R . Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from A59 in beta-glucan conversion. Biotechnol Rep (Amst). 2020; 28:e00526. PMC: 7490527. DOI: 10.1016/j.btre.2020.e00526. View

Grady E, MacDonald J, Liu L, Richman A, Yuan Z . Current knowledge and perspectives of Paenibacillus: a review. Microb Cell Fact. 2016; 15(1):203. PMC: 5134293. DOI: 10.1186/s12934-016-0603-7. View

10.

Wang L, Zhang Y, Gao P, Shi D, Liu H, Gao H . Changes in the structural properties and rate of hydrolysis of cotton fibers during extended enzymatic hydrolysis. Biotechnol Bioeng. 2005; 93(3):443-56. DOI: 10.1002/bit.20730. View

11.

Pahm A, Scherer C, Pettigrew J, Baker D, Parsons C, Stein H . Standardized amino acid digestibility in cecectomized roosters and lysine bioavailability in chicks fed distillers dried grains with solubles. Poult Sci. 2009; 88(3):571-8. DOI: 10.3382/ps.2008-00184. View

12.

Wang B, Chen K, Zhang P, Long L, Ding S . Comparison of the Biochemical Properties and Roles in the Xyloglucan-Rich Biomass Degradation of a GH74 Xyloglucanase and Its CBM-Deleted Variant from . Int J Mol Sci. 2022; 23(9). PMC: 9103125. DOI: 10.3390/ijms23095276. View

13.

Mukherjee S, Lodha T, Madhuprakash J . Comprehensive Genome Analysis of Cellulose and Xylan-Active CAZymes from the Genus : Special Emphasis on the Novel Xylanolytic sp. LS1. Microbiol Spectr. 2023; 11(3):e0502822. PMC: 10269863. DOI: 10.1128/spectrum.05028-22. View

14.

Brown M, Chang M . Exploring bacterial lignin degradation. Curr Opin Chem Biol. 2014; 19:1-7. DOI: 10.1016/j.cbpa.2013.11.015. View

15.

Leite P, Salgado J, Venancio A, Dominguez J, Belo I . Ultrasounds pretreatment of olive pomace to improve xylanase and cellulase production by solid-state fermentation. Bioresour Technol. 2016; 214:737-746. DOI: 10.1016/j.biortech.2016.05.028. View

16.

Basen M, Rhaesa A, Kataeva I, Prybol C, Scott I, Poole F . Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophilic bacterium Caldicellulosiruptor bescii. Bioresour Technol. 2013; 152:384-92. DOI: 10.1016/j.biortech.2013.11.024. View

17.

Sabathe F, Belaich A, Soucaille P . Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. FEMS Microbiol Lett. 2002; 217(1):15-22. DOI: 10.1111/j.1574-6968.2002.tb11450.x. View

18.

Chhe C, Uke A, Baramee S, Ungkulpasvich U, Tachaapaikoon C, Pason P . Draft genome sequence data of the facultative, thermophilic, xylanolytic bacterium sp. strain DA-C8. Data Brief. 2021; 35:106784. PMC: 7859314. DOI: 10.1016/j.dib.2021.106784. View

19.

Chen X, Ishida N, Todaka N, Nakamura R, Maruyama J, Takahashi H . Promotion of efficient Saccharification of crystalline cellulose by Aspergillus fumigatus Swo1. Appl Environ Microbiol. 2010; 76(8):2556-61. PMC: 2849193. DOI: 10.1128/AEM.02499-09. View

20.

Arntzen M, Pedersen B, Klau L, Stokke R, Oftebro M, Antonsen S . Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase. Appl Environ Microbiol. 2021; 87(6). PMC: 8105002. DOI: 10.1128/AEM.02399-20. View