Assessment of Lactobacillus Rhamnosus Mediated Protection Against Arsenic-induced Toxicity in Zebrafish: a QPCR-based Analysis of Firmicutes and Bacteroidetes Groups and Embryonic Development

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 2023 Aug 22

PMID 37608161

Authors

Abhishek Jain

Roshni Jain

Subodh Kumar Jain

Affiliations

Soon will be listed here.

Abstract

Arsenic poses a significant health risk worldwide, impacting the gut microbiota, reproductive health, and development. To address this issue, a cost-effective method like probiotic supplementation could be beneficial. However, the interplay between arsenic toxicity, probiotics, gut microbiota, and maternal transcript modulation remains unexplored. This study investigates the impact of Lactobacillus rhamnosus (L. rhamnosus) DSM 20021 on the proportions of Firmicutes and Bacteroidetes, as well as its effects on embryonic development in zebrafish induced by arsenic trioxide (AsO). Adult zebrafish were exposed to both high and environmentally relevant concentrations of AsO (10, 50, and 500 ppb) for 1, 6, and 12 weeks. qPCR analysis revealed increased proportions of Firmicutes and Bacteroidetes in all AsO-exposed and AsO + L. rhamnosus-exposed groups, while no significant changes were observed in groups exposed only to L. rhamnosus DSM 20021. The larvae, exposed to 500 ppb of AsO for 12 weeks, exhibited low growth, decreased survival rates, and morphological deformities. However, these adverse effects were reversed upon exposure to only L. rhamnosus DSM 20021. Furthermore, the expression of DVR1 and ABCC5, which are involved in defense against xenobiotics and embryo development, decreased significantly in AsO (500 ppb) and AsO (500 ppb) + L. rhamnosus-exposed groups, whereas ameliorative effects were observed in only L. rhamnosus DSM 20021-exposed groups.

Citing Articles

Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli.

Corrales D, Alcantara C, Clemente M, Velez D, Devesa V, Monedero V Int J Mol Sci. 2024; 25(9).

PMID: 38732236 PMC: 11084836. DOI: 10.3390/ijms25095017.

References

Abdel-Megeed R . Probiotics: a Promising Generation of Heavy Metal Detoxification. Biol Trace Elem Res. 2020; 199(6):2406-2413. DOI: 10.1007/s12011-020-02350-1. View

Allcock G, Allegra M, Flower R, Perretti M . Neutrophil accumulation induced by bacterial lipopolysaccharide: effects of dexamethasone and annexin 1. Clin Exp Immunol. 2001; 123(1):62-7. PMC: 1905950. DOI: 10.1046/j.1365-2249.2001.01370.x. View

Armstrong C, Stroube R, Rubio T, Siudyla E, Miller Jr G . Outbreak of fatal arsenic poisoning caused by contaminated drinking water. Arch Environ Health. 1984; 39(4):276-9. DOI: 10.1080/00039896.1984.10545849. View

Coral J, Heaps S, Glaholt S, Karty J, Jacobson S, Shaw J . Arsenic exposure induces a bimodal toxicity response in zebrafish. Environ Pollut. 2021; 287:117637. DOI: 10.1016/j.envpol.2021.117637. View

Coryell M, McAlpine M, Pinkham N, McDermott T, Walk S . The gut microbiome is required for full protection against acute arsenic toxicity in mouse models. Nat Commun. 2018; 9(1):5424. PMC: 6303300. DOI: 10.1038/s41467-018-07803-9. View

Dohrmann C, Kessler D, Melton D . Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1. Dev Biol. 1996; 175(1):108-17. DOI: 10.1006/dbio.1996.0099. View

Donato K, Gareau M, Wang Y, Sherman P . Lactobacillus rhamnosus GG attenuates interferon-{gamma} and tumour necrosis factor-alpha-induced barrier dysfunction and pro-inflammatory signalling. Microbiology (Reading). 2010; 156(Pt 11):3288-3297. DOI: 10.1099/mic.0.040139-0. View

Fierer N, Jackson J, Vilgalys R, Jackson R . Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol. 2005; 71(7):4117-20. PMC: 1169028. DOI: 10.1128/AEM.71.7.4117-4120.2005. View

Gagliardi A, Totino V, Cacciotti F, Iebba V, Neroni B, Bonfiglio G . Rebuilding the Gut Microbiota Ecosystem. Int J Environ Res Public Health. 2018; 15(8). PMC: 6121872. DOI: 10.3390/ijerph15081679. View

10.

Gao B, Chi L, Mahbub R, Bian X, Tu P, Ru H . Multi-Omics Reveals that Lead Exposure Disturbs Gut Microbiome Development, Key Metabolites, and Metabolic Pathways. Chem Res Toxicol. 2017; 30(4):996-1005. PMC: 5654721. DOI: 10.1021/acs.chemrestox.6b00401. View

11.

Gibiino G, Lopetuso L, Scaldaferri F, Rizzatti G, Binda C, Gasbarrini A . Exploring Bacteroidetes: Metabolic key points and immunological tricks of our gut commensals. Dig Liver Dis. 2018; 50(7):635-639. DOI: 10.1016/j.dld.2018.03.016. View

12.

Gioacchini G, Maradonna F, Lombardo F, Bizzaro D, Olivotto I, Carnevali O . Increase of fecundity by probiotic administration in zebrafish (Danio rerio). Reproduction. 2010; 140(6):953-9. DOI: 10.1530/REP-10-0145. View

13.

Guo X, Xia X, Tang R, Zhou J, Zhao H, Wang K . Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Lett Appl Microbiol. 2009; 47(5):367-73. DOI: 10.1111/j.1472-765X.2008.02408.x. View

14.

Kool M, de Haas M, Scheffer G, Scheper R, Van Eijk M, Juijn J . Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 1997; 57(16):3537-47. View

15.

Kuo C, Howard B, Umans J, Gribble M, Best L, Francesconi K . Arsenic Exposure, Arsenic Metabolism, and Incident Diabetes in the Strong Heart Study. Diabetes Care. 2015; 38(4):620-7. PMC: 4370323. DOI: 10.2337/dc14-1641. View

16.

Li X, Ma Y, Li D, Gao X, Li P, Bai N . Arsenic impairs embryo development via down-regulating Dvr1 expression in zebrafish. Toxicol Lett. 2012; 212(2):161-8. DOI: 10.1016/j.toxlet.2012.05.011. View

17.

Long Y, Li Q, Li J, Cui Z . Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish. Comp Biochem Physiol B Biochem Mol Biol. 2010; 158(1):46-55. DOI: 10.1016/j.cbpb.2010.09.005. View

18.

Lu K, Abo R, Schlieper K, Graffam M, Levine S, Wishnok J . Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis. Environ Health Perspect. 2014; 122(3):284-91. PMC: 3948040. DOI: 10.1289/ehp.1307429. View

19.

Lu K, Mahbub R, Cable P, Ru H, Parry N, Bodnar W . Gut microbiome phenotypes driven by host genetics affect arsenic metabolism. Chem Res Toxicol. 2014; 27(2):172-4. PMC: 3997221. DOI: 10.1021/tx400454z. View

20.

Lu Y, Zhang J, Yi H, Zhang Z, Zhang L . Screening of intestinal peristalsis-promoting probiotics based on a zebrafish model. Food Funct. 2019; 10(4):2075-2082. DOI: 10.1039/c8fo02523a. View