Dietary Supplementation of VA Enhances Growth, Feed Utilization, Glucose and Lipid Metabolism, Appetite, and Antioxidant Capacity of Chinese Perch (Siniperca Chuatsi)

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2023 Aug 18

PMID 37594622

Authors

Junliang Chen

Ming Zeng

Xu-Fang Liang

Di Peng

Ruipeng Xie

Dongliang Wu

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the effect of dietary vitamin A on juvenile Chinese perch (Siniperca chuatsi). Chinese perch were fed with five experimental diets containing 0, 20, 40, 60, and 80 mg VA·kg for 8 weeks. Results showed that dietary vitamin A significantly influenced the fish's growth, feed utilization, glucose and lipid metabolism, appetite, and antioxidant capacity. Vitamin A-supplemented groups had higher weight gain rate (WGR) and specific growth rate (SGR) compared to the control group. Feed conversion ratio (FCR) was also lower in the vitamin A-supplemented groups. Dietary vitamin A had no significant effect on the survival rate (SR). Compared to the control group, fish fed with vitamin A had increased feed intake (FI), and the expression of appetite-promoting genes (npy and agrp) was significantly higher in the 40 mg VA·kg group. Vitamin A also enhanced the utilization of dietary protein by Chinese perch. The serum glucose content of the fish fed with 40 mg VA·kg diet was significantly higher than that of the control group and 20 mg VA·kg diet, indicating that the promoting effect of VA on gluconeogenesis was greater than that on glycolysis. Additionally, dietary vitamin A increased the expression of lipid metabolism-related genes (hl and fas) and antioxidant genes (nrf2 and gpx) in the fish. These results suggest that the optimal vitamin A requirement of juvenile Chinese perch bream was estimated to be 37.32 mg VA·kg based on broken-line regression analysis of WGR. In conclusion, this study provides valuable insights into the potential benefits of dietary vitamin A on the growth, metabolism, and antioxidant capacity of Chinese perch.

Citing Articles

Transcriptome Sequencing Reveals Effects of Artificial Feed Domestication on Intestinal Performance and Gene Expression of Carnivorous Mandarin Fish (Siniperca chuatsi) and Related Mechanisms.

Luo J, Gao X, Rong Z, Zhang L, Sun Y, Qi Z Mar Biotechnol (NY). 2025; 27(1):41.

PMID: 39891779 DOI: 10.1007/s10126-025-10420-5.

References

Ahwach S, Thomas M, Onstead-Haas L, Mooradian A, Haas M . The glutathione mimic ebselen inhibits oxidative stress but not endoplasmic reticulum stress in endothelial cells. Life Sci. 2015; 134:9-15. DOI: 10.1016/j.lfs.2015.05.004. View

Berry D, Noy N . Signaling by vitamin A and retinol-binding protein in regulation of insulin responses and lipid homeostasis. Biochim Biophys Acta. 2011; 1821(1):168-76. PMC: 3204314. DOI: 10.1016/j.bbalip.2011.07.002. View

Blomhoff R, Blomhoff H . Overview of retinoid metabolism and function. J Neurobiol. 2006; 66(7):606-30. DOI: 10.1002/neu.20242. View

Bruzelius K, Sundler R, Pagmantidis V, Akesson B . Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells. J Trace Elem Med Biol. 2010; 24(4):251-6. DOI: 10.1016/j.jtemb.2010.08.001. View

Chen X, Kunsch C . Induction of cytoprotective genes through Nrf2/antioxidant response element pathway: a new therapeutic approach for the treatment of inflammatory diseases. Curr Pharm Des. 2004; 10(8):879-91. DOI: 10.2174/1381612043452901. View

Clagett-Dame M, Deluca H . The role of vitamin A in mammalian reproduction and embryonic development. Annu Rev Nutr. 2002; 22:347-81. DOI: 10.1146/annurev.nutr.22.010402.102745E. View

Costas B, Aragao C, Ruiz-Jarabo I, Vargas-Chacoff L, Arjona F, Dinis M . Feed deprivation in Senegalese sole (Solea senegalensis Kaup, 1858) juveniles: effects on blood plasma metabolites and free amino acid levels. Fish Physiol Biochem. 2010; 37(3):495-504. DOI: 10.1007/s10695-010-9451-2. View

DAmbrosio D, Clugston R, Blaner W . Vitamin A metabolism: an update. Nutrients. 2011; 3(1):63-103. PMC: 3042718. DOI: 10.3390/nu3010063. View

Enes P, Panserat S, Kaushik S, Oliva-Teles A . Nutritional regulation of hepatic glucose metabolism in fish. Fish Physiol Biochem. 2008; 35(3):519-39. DOI: 10.1007/s10695-008-9259-5. View

10.

Gaspar C, Silva-Marrero J, Fabregas A, Minarro M, Tico J, Baanante I . Administration of chitosan-tripolyphosphate-DNA nanoparticles to knockdown glutamate dehydrogenase expression impairs transdeamination and gluconeogenesis in the liver. J Biotechnol. 2018; 286:5-13. DOI: 10.1016/j.jbiotec.2018.09.002. View

11.

He Y, Gong L, Fang Y, Zhan Q, Liu H, Lu Y . The role of retinoic acid in hepatic lipid homeostasis defined by genomic binding and transcriptome profiling. BMC Genomics. 2013; 14:575. PMC: 3846674. DOI: 10.1186/1471-2164-14-575. View

12.

HUDSON R, Daniel R . L-glutamate dehydrogenases: distribution, properties and mechanism. Comp Biochem Physiol B. 1993; 106(4):767-92. DOI: 10.1016/0305-0491(93)90031-y. View

13.

Jeyakumar S, Sheril A, Vajreswari A . Vitamin A Improves Hyperglycemia and Glucose-Intolerance through Regulation of Intracellular Signaling Pathways and Glycogen Synthesis in WNIN/GR-Ob Obese Rat Model. Prev Nutr Food Sci. 2017; 22(3):172-183. PMC: 5642798. DOI: 10.3746/pnf.2017.22.3.172. View

14.

Joseph S, Laffitte B, Patel P, Watson M, Matsukuma K, Walczak R . Direct and indirect mechanisms for regulation of fatty acid synthase gene expression by liver X receptors. J Biol Chem. 2002; 277(13):11019-25. DOI: 10.1074/jbc.M111041200. View

15.

Klyuyeva A, Belyaeva O, Goggans K, Krezel W, Popov K, Kedishvili N . Changes in retinoid metabolism and signaling associated with metabolic remodeling during fasting and in type I diabetes. J Biol Chem. 2021; 296:100323. PMC: 7949101. DOI: 10.1016/j.jbc.2021.100323. View

16.

Lamb T, Pugh Jr E . Dark adaptation and the retinoid cycle of vision. Prog Retin Eye Res. 2004; 23(3):307-80. DOI: 10.1016/j.preteyeres.2004.03.001. View

17.

Li Y, Li R, Chen W, Chen G . Vitamin A status and its metabolism contribute to the regulation of hepatic genes during the cycle of fasting and refeeding in rats. J Nutr Biochem. 2016; 30:33-43. DOI: 10.1016/j.jnutbio.2015.11.012. View

18.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

19.

Mangelsdorf D, Evans R . The RXR heterodimers and orphan receptors. Cell. 1995; 83(6):841-50. DOI: 10.1016/0092-8674(95)90200-7. View

20.

Manolescu D, Sima A, Bhat P . All-trans retinoic acid lowers serum retinol-binding protein 4 concentrations and increases insulin sensitivity in diabetic mice. J Nutr. 2009; 140(2):311-6. DOI: 10.3945/jn.109.115147. View