Behavior of Deteriogenic Fungi in Aviation Fuels (fossil and Biofuel) During Simulated Storage

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2023 Aug 16

PMID 37584891

Authors

Mariane Rodrigues Lobato

Juciana Clarice Cazarolli

Regiane Debora Fernandes Rios

Emmanuel Bezerra D Alessandro

Marcia T S Lutterbach

Nelson Roberto Antoniosi Filho

Vanya Marcia Duarte Pasa

Donato Aranda

Pedro Rodrigo Scorza

Fatima Menezes Bento

Affiliations

Soon will be listed here.

Abstract

Biofuels are expected to play a major role in reducing carbon emissions in the aviation sector globally. Farnesane ("2,6,10-trimethyldodecane") is a biofuel derived from the synthesized iso-paraffin route wich can be blended with jet fuel; however, the microbial behavior in farnesane/jet fuel blends remains unknown. The chemical and biological stability of blends should be investigated to ensure they meet the quality requirements for aviation fuels. This work aimed at evaluating the behavior of two fungi Hormoconis resinae (F089) and Exophiala phaeomuriformis (UFRGS Q4.2) in jet fuel, farnesane, and in 10% farnesane blend during simulated storage. Microcosms (150-mL flasks) were assembled with and without fungi containing Bushnell & Haas mineral medium for 28 days at a temperature of 20±2°C. The fungal growth (biomass), pH, surface tension, and changes in the fuel's hydrocarbon chains were evaluated. This study revealed thatthe treatment containing H. resinae showed a biomass of 19 mg, 12 mg, and 2 mg for jet fuel, blend, and farnesane respectively. The pH was reduced from 7.2 to 4.3 observed in jet fuel treatment The degradation results showed that compounds with carbon chains between C and C, in jet fuel, and blend treatments were preferably degraded. The highest biomass (70.9 mg) produced by E. phaeomuriformis was in 10% farnesane blend, after 21 days. However, no significant decrease was observed on pH and surface tension measurements across the treatments as well as on the hydrocarbons when compared to the controls. This study revealed that farnesane neither inhibited nor promoted greater growth on both microorganisms.

References

Martin-Sanchez P, Gorbushina A, Kunte H, Toepel J . A novel qPCR protocol for the specific detection and quantification of the fuel-deteriorating fungus Hormoconis resinae. Biofouling. 2016; 32(6):635-44. DOI: 10.1080/08927014.2016.1177515. View

Rauch M, Graef H, Rozenzhak S, Sharon E Jones , Bleckmann C, Kruger R . Characterization of microbial contamination in United States Air Force aviation fuel tanks. J Ind Microbiol Biotechnol. 2005; 33(1):29-36. DOI: 10.1007/s10295-005-0023-x. View

White J, Gilbert J, Hill G, Hill E, Huse S, Weightman A . Culture-independent analysis of bacterial fuel contamination provides insight into the level of concordance with the standard industry practice of aerobic cultivation. Appl Environ Microbiol. 2011; 77(13):4527-38. PMC: 3127687. DOI: 10.1128/AEM.02317-10. View

Buddie A, Bridge P, Kelley J, Ryan M . Candida keroseneae sp. nov., a novel contaminant of aviation kerosene. Lett Appl Microbiol. 2010; 52(1):70-5. DOI: 10.1111/j.1472-765X.2010.02968.x. View

Bushnell L, Haas H . The Utilization of Certain Hydrocarbons by Microorganisms. J Bacteriol. 1941; 41(5):653-73. PMC: 374727. DOI: 10.1128/jb.41.5.653-673.1941. View

Ergin C, Gok Y, Baygu Y, Gumral R, Ozhak-Baysan B, Dogen A . ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS. Microb Ecol. 2015; 71(2):339-46. DOI: 10.1007/s00248-015-0670-z. View

Isola D, Selbmann L, de Hoog G, Fenice M, Onofri S, Prenafeta-Boldu F . Isolation and screening of black fungi as degraders of volatile aromatic hydrocarbons. Mycopathologia. 2013; 175(5-6):369-79. DOI: 10.1007/s11046-013-9635-2. View

Zalar P, Novak M, de Hoog G, Gunde-Cimerman N . Dishwashers--a man-made ecological niche accommodating human opportunistic fungal pathogens. Fungal Biol. 2011; 115(10):997-1007. DOI: 10.1016/j.funbio.2011.04.007. View

Matos T, Haase G, Gerrits van den Ende A, de Hoog G . Molecular diversity of oligotrophic and neurotropic members of the black yeast genus Exophiala, with accent on E. dermatitidis. Antonie Van Leeuwenhoek. 2003; 83(4):293-303. DOI: 10.1023/a:1023373329502. View

10.

Najafzadeh M, Dolatabadi S, Keisari M, Naseri A, Feng P, de Hoog G . Detection and identification of opportunistic Exophiala species using the rolling circle amplification of ribosomal internal transcribed spacers. J Microbiol Methods. 2013; 94(3):338-42. DOI: 10.1016/j.mimet.2013.06.026. View

11.

Siporin C, Cooney J . Inhibition of glucose metabolism by n-hexadecane in Cladosporium (Amorphotheca) resinae. J Bacteriol. 1976; 128(1):235-41. PMC: 232848. DOI: 10.1128/jb.128.1.235-241.1976. View

12.

Seifert K, Hughes S, Boulay H, Louis-Seize G . Taxonomy, nomenclature and phylogeny of three cladosporium-like hyphomycetes, Sorocybe resinae, Seifertia azaleae and the Hormoconis anamorph of Amorphotheca resinae. Stud Mycol. 2008; 58:235-45. PMC: 2104743. DOI: 10.3114/sim.2007.58.09. View

13.

Edmonds P, Cooney J . Identification of microorganisms isolated from jet fuel systems. Appl Microbiol. 1967; 15(2):411-6. PMC: 546914. DOI: 10.1128/am.15.2.411-416.1967. View

14.

Cofone Jr L, Walker J, Cooney J . Utilization of hydrocarbons by Cladosporium resinae. J Gen Microbiol. 1973; 76(1):243-6. DOI: 10.1099/00221287-76-1-243. View

15.

Cooney J, Proby C . Fatty acid composition of Cladosporium resinae grown on glucose and on hydrocarbons. J Bacteriol. 1971; 108(2):777-81. PMC: 247140. DOI: 10.1128/jb.108.2.777-781.1971. View

16.

Teh J, Lee K . Utilization of n-alkanes by Cladosporium resinae. Appl Microbiol. 1973; 25(3):454-7. PMC: 380827. DOI: 10.1128/am.25.3.454-457.1973. View

17.

Kaczorek E, Jesionowski T, Giec A, Olszanowski A . Cell surface properties of Pseudomonas stutzeri in the process of diesel oil biodegradation. Biotechnol Lett. 2012; 34(5):857-62. PMC: 3349024. DOI: 10.1007/s10529-011-0835-x. View

18.

Zhang Y, Miller R . Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl Environ Microbiol. 1992; 58(10):3276-82. PMC: 183091. DOI: 10.1128/aem.58.10.3276-3282.1992. View

19.

Khan S, Nirmal J, Kumar R, Patel J . Biodegradation of kerosene: Study of growth optimization and metabolic fate of P. janthinellum SDX7. Braz J Microbiol. 2015; 46(2):397-406. PMC: 4507531. DOI: 10.1590/S1517-838246220140112. View

20.

Walker J, Cooney J . Pathway of n-alkane oxidation in Cladosporium resinae. J Bacteriol. 1973; 115(2):635-9. PMC: 246293. DOI: 10.1128/jb.115.2.635-639.1973. View