Identification of Novel Antimicrobial Peptides from the Venom Gland Transcriptome of the Spider (Scopoli, 1772)

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Aug 14

PMID 37577428

Authors

Min Kyoung Shin

In-Wook Hwang

Bo-Young Jang

Kyung-Bin Bu

Jung Sun Yoo

Jung-Suk Sung

Affiliations

Soon will be listed here.

Abstract

As the emergence and prevalence of antibiotic-resistant strains have resulted in a global crisis, there is an urgent need for new antimicrobial agents. Antimicrobial peptides (AMPs) exhibit inhibitory activity against a wide spectrum of pathogens and can be utilized as an alternative to conventional antibiotics. In this study, two novel AMPs were identified from the venom transcriptome of the spider (Scopoli, 1772) using methods, and their antimicrobial activity was experimentally validated. Aranetoxin-Ab2a (AATX-Ab2a) and Aranetoxin-Ab3a (AATX-Ab3a) were identified by homology analysis and were predicted to have high levels of antimicrobial activity based on analysis. Both peptides were found to have antibacterial effect against Gram-positive and -negative strains, and, in particular, showed significant inhibitory activity against multidrug-resistant isolates. In addition, AATX-Ab2a and AATX-Ab3a inhibited animal and vegetable fungal strains, while showing low toxicity to normal human cells. The antimicrobial activity of the peptides was attributed to the increased permeability of microbial membranes. The study described the discovery of novel antibiotic candidates, AATX-Ab2a and AATX-Ab3a, using the spider venom gland transcriptome, and validated an -based method for identifying functional substances from biological resources.

Citing Articles

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References

Yount N, Bayer A, Xiong Y, Yeaman M . Advances in antimicrobial peptide immunobiology. Biopolymers. 2006; 84(5):435-58. DOI: 10.1002/bip.20543. View

Yacoub T, Rima M, Karam M, Fajloun J . Antimicrobials from Venomous Animals: An Overview. Molecules. 2020; 25(10). PMC: 7287856. DOI: 10.3390/molecules25102402. View

Kalelkar P, Riddick M, Garcia A . Biomaterial-based delivery of antimicrobial therapies for the treatment of bacterial infections. Nat Rev Mater. 2022; 7(1):39-54. PMC: 8938918. DOI: 10.1038/s41578-021-00362-4. View

Utkin Y . Animal venom studies: Current benefits and future developments. World J Biol Chem. 2015; 6(2):28-33. PMC: 4436903. DOI: 10.4331/wjbc.v6.i2.28. View

Schmidt B, Hildebrandt A . Deep learning in next-generation sequencing. Drug Discov Today. 2020; 26(1):173-180. PMC: 7550123. DOI: 10.1016/j.drudis.2020.10.002. View

Lewis R, Garcia M . Therapeutic potential of venom peptides. Nat Rev Drug Discov. 2003; 2(10):790-802. DOI: 10.1038/nrd1197. View

Bahar A, Ren D . Antimicrobial peptides. Pharmaceuticals (Basel). 2013; 6(12):1543-75. PMC: 3873676. DOI: 10.3390/ph6121543. View

Sato H, Feix J . Peptide-membrane interactions and mechanisms of membrane destruction by amphipathic alpha-helical antimicrobial peptides. Biochim Biophys Acta. 2006; 1758(9):1245-56. DOI: 10.1016/j.bbamem.2006.02.021. View

Sitaram N, Nagaraj R . Host-defense antimicrobial peptides: importance of structure for activity. Curr Pharm Des. 2002; 8(9):727-42. DOI: 10.2174/1381612023395358. View

10.

Mwangi J, Hao X, Lai R, Zhang Z . Antimicrobial peptides: new hope in the war against multidrug resistance. Zool Res. 2019; 40(6):488-505. PMC: 6822926. DOI: 10.24272/j.issn.2095-8137.2019.062. View

11.

Larsson D, Flach C . Antibiotic resistance in the environment. Nat Rev Microbiol. 2021; 20(5):257-269. PMC: 8567979. DOI: 10.1038/s41579-021-00649-x. View

12.

Wang X, Wang G . Insights into Antimicrobial Peptides from Spiders and Scorpions. Protein Pept Lett. 2016; 23(8):707-21. PMC: 4967028. DOI: 10.2174/0929866523666160511151320. View

13.

Cardoso M, Orozco R, Rezende S, Rodrigues G, Oshiro K, Candido E . Computer-Aided Design of Antimicrobial Peptides: Are We Generating Effective Drug Candidates?. Front Microbiol. 2020; 10:3097. PMC: 6987251. DOI: 10.3389/fmicb.2019.03097. View

14.

Roly Z, Islam M, Reza M . A comparative in silico characterization of functional and physicochemical properties of 3FTx (three finger toxin) proteins from four venomous snakes. Bioinformation. 2014; 10(5):281-7. PMC: 4070037. DOI: 10.6026/97320630010281. View

15.

Vamathevan J, Clark D, Czodrowski P, Dunham I, Ferran E, Lee G . Applications of machine learning in drug discovery and development. Nat Rev Drug Discov. 2019; 18(6):463-477. PMC: 6552674. DOI: 10.1038/s41573-019-0024-5. View

16.

Shin M, Hwang I, Kim Y, Kim S, Jang W, Lee S . Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider . Antibiotics (Basel). 2020; 9(7). PMC: 7400607. DOI: 10.3390/antibiotics9070422. View

17.

Saez N, Senff S, Jensen J, Er S, Herzig V, Rash L . Spider-venom peptides as therapeutics. Toxins (Basel). 2011; 2(12):2851-71. PMC: 3153181. DOI: 10.3390/toxins2122851. View

18.

Rastogi S, Shukla S, Kalaivani M, Singh G . Peptide-based therapeutics: quality specifications, regulatory considerations, and prospects. Drug Discov Today. 2018; 24(1):148-162. DOI: 10.1016/j.drudis.2018.10.002. View

19.

Melo M, Ferre R, Castanho M . Antimicrobial peptides: linking partition, activity and high membrane-bound concentrations. Nat Rev Microbiol. 2009; 7(3):245-50. DOI: 10.1038/nrmicro2095. View

20.

Aslam B, Khurshid M, Arshad M, Muzammil S, Rasool M, Yasmeen N . Antibiotic Resistance: One Health One World Outlook. Front Cell Infect Microbiol. 2021; 11:771510. PMC: 8656695. DOI: 10.3389/fcimb.2021.771510. View