Diversity of Transgenes in Sustainable Management of Insect Pests

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 2023 Aug 12

PMID 37573273

Authors

V Rakesh

Vinay K Kalia

Amalendu Ghosh

Affiliations

Soon will be listed here.

Abstract

Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with the natural defenses developed by plants to counter insect attacks. Several toxin genes are also derived from spiders and scorpions for protection against insects. Bacillus thuringiensis Berliner is a microbial source of insecticidal toxins. Several methods have facilitated the large-scale production of transgenic plants. Bt-derived cry, cyt, vip, and sip genes, plant-derived genes such as lectins, protease inhibitors, and alpha-amylase inhibitors, insect cell wall-degrading enzymes like chitinase and some proteins like arcelins, plant defensins, and ribosome-inactivating proteins have been successfully utilized to impart resistance to insects. Besides, transgenic plants expressing double-stranded RNA have been developed with enhanced resistance. However, the long-term effects of transgenes on insect resistance, the environment, and human health must be thoroughly investigated before they are made available for commercial planting. In this chapter, the present status, prospects, and future scope of transgenes for insect pest management have been summarized and discussed.

Citing Articles

Resistance of Populus davidiana × P. bolleana overexpressing cinnamoyl-CoA reductase gene to Lymantria dispar larvae.

Li Y, Zhang R, Sun L, Cao C Transgenic Res. 2025; 34(1):10.

PMID: 39786661 DOI: 10.1007/s11248-024-00426-5.

Advancements in genetically modified insect pest-resistant crops in India.

Rakesh V, Ghosh A Planta. 2024; 260(4):86.

PMID: 39230667 DOI: 10.1007/s00425-024-04511-1.

Insect α-Amylases and Their Application in Pest Management.

Wang B, Huang D, Cao C, Gong Y Molecules. 2023; 28(23).

PMID: 38067617 PMC: 10708458. DOI: 10.3390/molecules28237888.

References

Agra-Neto A, Napoleao T, Pontual E, Santos N, de Andrade Luz L, Oliveira C . Effect of Moringa oleifera lectins on survival and enzyme activities of Aedes aegypti larvae susceptible and resistant to organophosphate. Parasitol Res. 2013; 113(1):175-84. DOI: 10.1007/s00436-013-3640-8. View

Alves R, Soares T, Bento E, Roldan-Filho R, Souza B, Lima M . Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs. Pest Manag Sci. 2019; 76(2):730-736. DOI: 10.1002/ps.5572. View

Aoki K, Perlman M, Lim J, Cantu R, Wells L, Tiemeyer M . Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo. J Biol Chem. 2007; 282(12):9127-42. DOI: 10.1074/jbc.M606711200. View

Arakane Y, Muthukrishnan S . Insect chitinase and chitinase-like proteins. Cell Mol Life Sci. 2009; 67(2):201-16. PMC: 11115512. DOI: 10.1007/s00018-009-0161-9. View

Barrett M, Udani J . A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): a review of clinical studies on weight loss and glycemic control. Nutr J. 2011; 10:24. PMC: 3071778. DOI: 10.1186/1475-2891-10-24. View

Bertholdo-Vargas L, Martins J, Bordin D, Salvador M, Schafer A, de Barros N . Type 1 ribosome-inactivating proteins - entomotoxic, oxidative and genotoxic action on Anticarsia gemmatalis (Hübner) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). J Insect Physiol. 2008; 55(1):51-8. DOI: 10.1016/j.jinsphys.2008.10.004. View

Bhavya J, Francois N, More V, More S . Scorpion Toxin Polyptides as Therapeutic Agents: An Overview. Protein Pept Lett. 2016; 23(9):848-59. DOI: 10.2174/0929866523666160630184635. View

Boddupally D, Tamirisa S, Gundra S, Vudem D, Khareedu V . Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests. Sci Rep. 2018; 8(1):8458. PMC: 5981619. DOI: 10.1038/s41598-018-26881-9. View

Bown D, Wilkinson H, Gatehouse J . Differentially regulated inhibitor-sensitive and insensitive protease genes from the phytophagous insect pest, Helicoverpa armigera, are members of complex multigene families. Insect Biochem Mol Biol. 1997; 27(7):625-38. DOI: 10.1016/s0965-1748(97)00043-x. View

10.

Bravo A, Likitvivatanavong S, Gill S, Soberon M . Bacillus thuringiensis: A story of a successful bioinsecticide. Insect Biochem Mol Biol. 2011; 41(7):423-31. PMC: 3689885. DOI: 10.1016/j.ibmb.2011.02.006. View

11.

Broadway R . Dietary regulation of serine proteinases that are resistant to serine proteinase inhibitors. J Insect Physiol. 2003; 43(9):855-874. DOI: 10.1016/s0022-1910(97)00028-0. View

12.

Brown S, Cao A, Dobson P, Hines E, Akhurst R, East P . Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria. Appl Environ Microbiol. 2006; 72(2):1653-62. PMC: 1392922. DOI: 10.1128/AEM.72.2.1653-1662.2006. View

13.

Burkness E, Dively G, Patton T, Morey A, Hutchison W . Novel Vip3A Bacillus thuringiensis (Bt) maize approaches high-dose efficacy against Helicoverpa zea (Lepidoptera: Noctuidae) under field conditions: Implications for resistance management. GM Crops. 2011; 1(5):337-43. DOI: 10.4161/gmcr.1.5.14765. View

14.

Butko P . Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypotheses. Appl Environ Microbiol. 2003; 69(5):2415-22. PMC: 154483. DOI: 10.1128/AEM.69.5.2415-2422.2003. View

15.

Cattaneo M, Yafuso C, Schmidt C, Huang C, Rahman M, Olson C . Farm-scale evaluation of the impacts of transgenic cotton on biodiversity, pesticide use, and yield. Proc Natl Acad Sci U S A. 2006; 103(20):7571-6. PMC: 1457091. DOI: 10.1073/pnas.0508312103. View

16.

Chakraborty P, Ghosh A . Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Asia II 1. Cells. 2022; 11(5). PMC: 8909865. DOI: 10.3390/cells11050833. View

17.

Chakraborty M, Reddy P, Mustafa G, Rajesh G, Narasu V, Udayasuriyan V . Transgenic rice expressing the cry2AX1 gene confers resistance to multiple lepidopteran pests. Transgenic Res. 2016; 25(5):665-78. DOI: 10.1007/s11248-016-9954-4. View

18.

Chakroun M, Banyuls N, Bel Y, Escriche B, Ferre J . Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria. Microbiol Mol Biol Rev. 2016; 80(2):329-50. PMC: 4867366. DOI: 10.1128/MMBR.00060-15. View

19.

Chavez-Mendoza C, Sanchez E . Bioactive Compounds from Mexican Varieties of the Common Bean (Phaseolus vulgaris): Implications for Health. Molecules. 2017; 22(8). PMC: 6152262. DOI: 10.3390/molecules22081360. View

20.

Mei F, Meng K, Gu Z, Yun Y, Zhang W, Zhang C . Arecanut ( L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats. J Agric Food Chem. 2020; 69(1):246-258. DOI: 10.1021/acs.jafc.0c06671. View