Combined Proteomics and Transcriptomics Identifies Serpin Family C Member 1 Associated Protein As a Biomarker of Endometriosis

Overview

Journal Ann Med

Publisher Informa Healthcare

Specialty General Medicine

Date 2023 Aug 12

PMID 37572646

Authors

Xiao-Yan Li

Xi Wang

Zhi-Yue Gu

Ting-Ting Sun

Jin-Hua Leng

Qi Yu

Affiliations

Soon will be listed here.

Abstract

Objective: To explore potential biomarkers indicating endometriosis (EM).

Materials And Methods: A proteomics method and combined quantitative transcriptomics were adopted to highlight markers in the EM. Venn analysis was used to integrate the ribonucleic acid sequencing (RNA-seq) and protein profiles. Promising candidate markers were tested by enzyme-related immunosorbent assay.

Results: A sum of 979 mRNAs and 39 proteins were tested to be significantly differentially expression in the standard cluster compared with the EM cluster. Venn analysis showed a filtered signature of only two down-regulated molecules in the EM group, i.e. fetuin B (FETUB) and serpin family C member 1 (SERPINC1); the latter showed a big variance between the control category and the EM set in the authentication test.

Conclusion: SERPINC1 may be a useful possible biomarker for the analysis of EM.

References

Blackburn K, Mbeunkui F, Mitra S, Mentzel T, Goshe M . Improving protein and proteome coverage through data-independent multiplexed peptide fragmentation. J Proteome Res. 2010; 9(7):3621-37. DOI: 10.1021/pr100144z. View

Zhai W, Li X, Wu S, Zhang Y, Pang H, Chen W . Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma. Int J Oncol. 2015; 46(5):2125-37. DOI: 10.3892/ijo.2015.2897. View

Siristatidis C . What have the 'omics done for endometriosis?. Med Sci Monit. 2009; 15(5):RA116-23. View

Yang L, Zhang J, Jiang A, Liu Q, Li C, Yang C . Expression profile of long non-coding RNAs is altered in endometrial cancer. Int J Clin Exp Med. 2015; 8(4):5010-21. PMC: 4483839. View

Nisenblat V, Bossuyt P, Shaikh R, Farquhar C, Jordan V, Scheffers C . Blood biomarkers for the non-invasive diagnosis of endometriosis. Cochrane Database Syst Rev. 2016; (5):CD012179. PMC: 7076288. DOI: 10.1002/14651858.CD012179. View

Ahmed W, Liu Z . Long Non-Coding RNAs: Novel Players in Regulation of Immune Response Upon Herpesvirus Infection. Front Immunol. 2018; 9:761. PMC: 5906719. DOI: 10.3389/fimmu.2018.00761. View

Taylor H, Kotlyar A, Flores V . Endometriosis is a chronic systemic disease: clinical challenges and novel innovations. Lancet. 2021; 397(10276):839-852. DOI: 10.1016/S0140-6736(21)00389-5. View

Gettins P . Serpin structure, mechanism, and function. Chem Rev. 2002; 102(12):4751-804. DOI: 10.1021/cr010170+. View

Zhao M, Wang J, Xi X, Tan N, Zhang L . SNHG12 Promotes Angiogenesis Following Ischemic Stroke via Regulating miR-150/VEGF Pathway. Neuroscience. 2018; 390:231-240. DOI: 10.1016/j.neuroscience.2018.08.029. View

10.

Fassbender A, Waelkens E, Verbeeck N, Kyama C, Bokor A, Vodolazkaia A . Proteomics analysis of plasma for early diagnosis of endometriosis. Obstet Gynecol. 2012; 119(2 Pt 1):276-85. DOI: 10.1097/AOG.0b013e31823fda8d. View

11.

May K, Villar J, Kirtley S, Kennedy S, Becker C . Endometrial alterations in endometriosis: a systematic review of putative biomarkers. Hum Reprod Update. 2011; 17(5):637-53. DOI: 10.1093/humupd/dmr013. View

12.

Aghajanova L, Giudice L . Molecular evidence for differences in endometrium in severe versus mild endometriosis. Reprod Sci. 2010; 18(3):229-51. PMC: 3118406. DOI: 10.1177/1933719110386241. View

13.

Bock S, Harris J, Balazs I, Trent J . Assignment of the human antithrombin III structural gene to chromosome 1q23-25. Cytogenet Cell Genet. 1985; 39(1):67-9. DOI: 10.1159/000132105. View

14.

Dietzel E, Wessling J, Floehr J, Schafer C, Ensslen S, Denecke B . Fetuin-B, a liver-derived plasma protein is essential for fertilization. Dev Cell. 2013; 25(1):106-12. DOI: 10.1016/j.devcel.2013.03.001. View

15.

Giudice L, Kao L . Endometriosis. Lancet. 2004; 364(9447):1789-99. DOI: 10.1016/S0140-6736(04)17403-5. View

16.

Wu X, Lu W, Xu C, Jiang C, Zhuo Z, Wang R . Macrophages Phenotype Regulated by IL-6 Are Associated with the Prognosis of Platinum-Resistant Serous Ovarian Cancer: Integrated Analysis of Clinical Trial and Omics. J Immunol Res. 2023; 2023:6455704. PMC: 10132904. DOI: 10.1155/2023/6455704. View

17.

Gomez R, Abad A, Delgado F, Tamarit S, Simon C, Pellicer A . Effects of hyperprolactinemia treatment with the dopamine agonist quinagolide on endometriotic lesions in patients with endometriosis-associated hyperprolactinemia. Fertil Steril. 2010; 95(3):882-8.e1. DOI: 10.1016/j.fertnstert.2010.10.024. View

18.

Wang X, Yu Q . Endometriosis-related ceRNA network to identify predictive biomarkers of endometrial receptivity. Epigenomics. 2019; 11(2):147-167. DOI: 10.2217/epi-2018-0190. View

19.

Zhang X, Xu Y, Fu L, Li D, Dai X, Liu L . Identification of mRNAs related to endometrium function regulated by lncRNA CD36-005 in rat endometrial stromal cells. Reprod Biol Endocrinol. 2018; 16(1):96. PMC: 6190555. DOI: 10.1186/s12958-018-0412-4. View

20.

Fassbender A, Verbeeck N, Bornigen D, Kyama C, Bokor A, Vodolazkaia A . Combined mRNA microarray and proteomic analysis of eutopic endometrium of women with and without endometriosis. Hum Reprod. 2012; 27(7):2020-9. DOI: 10.1093/humrep/des127. View