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Valorisation of Pineapple Cannery Waste As a Cost Effective Carbon Source for Poly 3-hydroxyabutyrate (P3HB) Production

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Publisher MDPI
Date 2023 Aug 12
PMID 37571191
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Abstract

Pineapple is one of the most important agro-industrial sugar-based fruits in Thailand. In this study, the waste stream from pineapple cannery processing was utilised and evaluated for potential use in the production of a main biopolymer group widely known as polyhydroxyalkanoates (PHAs) through aerobic batch fermentation. Firstly, pineapple cannery waste (PCW) collected from three processing sources, pineapple juice (PAJ), peel and core juice (PCJ), and pulp-washing water (PWW), was used as a carbon source. Secondly, it was characterised and pretreated. Then, batch fermentation was performed by using the optimal condition (200 rpm agitation rate, 37 °C, and fermentation time of 72 h) under two different nutrient conditions in each type of carbon source. The results revealed that PHAs were produced during 24-72 h of fermentation without any interference. The PHAs product obtained was characterised by their properties. Interestingly, GC-MS showed homopolymer of poly 3-hydroxybutyrate (P3HB) group characteristics, such as OH, CH, and C=O; meanwhile, H NMR analysis showed signals corresponding to CH, CH, and CH, respectively. Remarkably, utilising the PCW showed a high-potential cheap carbon source for the production of PHAs as well as for the treatment of wastewater from the fruit industry.

Citing Articles

Repeated Fed-Batch Culture Strategy for the Synthesis of Polyhydroxybutyrate (PHB) Biopolymers from Sugar Cane Juice Using .

Dujjanutat P, Singhaboot P, Kaewkannetra P Polymers (Basel). 2024; 16(22).

PMID: 39599246 PMC: 11598619. DOI: 10.3390/polym16223156.

References
1.
Abbas S, Shanbhag T, Kothare A . Applications of bromelain from pineapple waste towards acne. Saudi J Biol Sci. 2021; 28(1):1001-1009. PMC: 7785454. DOI: 10.1016/j.sjbs.2020.11.032. View

2.
Suwannasing W, Imai T, Kaewkannetra P . Cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials. Bioresour Technol. 2015; 194:67-74. DOI: 10.1016/j.biortech.2015.06.087. View

3.
Vega-Castro O, Contreras-Calderon J, Leon E, Segura A, Arias M, Perez L . Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha. J Biotechnol. 2016; 231:232-238. DOI: 10.1016/j.jbiotec.2016.06.018. View

4.
Park S, Kim T, Kim M, Lee S, Lim S . Advanced bacterial polyhydroxyalkanoates: towards a versatile and sustainable platform for unnatural tailor-made polyesters. Biotechnol Adv. 2011; 30(6):1196-206. DOI: 10.1016/j.biotechadv.2011.11.007. View

5.
Kostiuchenko O, Kravchenko N, Markus J, Burleigh S, Fedkiv O, Cao L . Effects of Proteases from Pineapple and Papaya on Protein Digestive Capacity and Gut Microbiota in Healthy C57BL/6 Mice and Dose-Manner Response on Mucosal Permeability in Human Reconstructed Intestinal 3D Tissue Model. Metabolites. 2022; 12(11). PMC: 9696696. DOI: 10.3390/metabo12111027. View