Understanding the Anti-Obesity Potential of an Avocado Oil-Rich Cheese Through an In Vitro Co-Culture Intestine Cell Model

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Aug 12

PMID 37570893

Authors

Manuela Machado

Eduardo M Costa

Sara Silva

Luis M Rodriguez-Alcala

Ana Maria Gomes

Manuela Pintado

Affiliations

Soon will be listed here.

Abstract

Nowadays, with consumers' requirements shifting towards more natural solutions and the advent of nutraceutical-based approaches, new alternatives for obesity management are being developed. This work aimed to show, for the first time, the potential of avocado oil-fortified cheese as a viable foodstuff for obesity management through complex in vitro cellular models. The results showed that oleic and palmitic acids' permeability through the Caco-2/HT29-MTX membrane peaked at the 2h mark, with the highest apparent permeability being registered for oleic acid (0.14 cm/s). Additionally, the permeated compounds were capable of modulating the metabolism of adipocytes present in the basal compartment, significantly reducing adipokine (leptin) and cytokine (MPC-1, IL-10, and TNF-α) production. The permeates (containing 3.30 µg/mL of palmitic acid and 2.16 µg/mL of oleic acid) also presented an overall anti-inflammatory activity upon Raw 264.7 macrophages, reducing IL-6 and TNF-α secretion. Despite in vivo assays being required, the data showed the potential of a functional dairy product as a valid food matrix to aid in obesity management.

Citing Articles

The Role of Unsaturated Fatty Acid-Rich Dairy Products in Adipocyte Metabolism.

Machado M, Costa E, Silva S, Gomes A, Pintado M Molecules. 2024; 29(23).

PMID: 39683663 PMC: 11643689. DOI: 10.3390/molecules29235502.

Enhancing Medium-Chain Fatty Acid Delivery Through Bigel Technology.

Machado M, Costa E, Silva S, Sousa S, Gomes A, Pintado M Gels. 2024; 10(11).

PMID: 39590094 PMC: 11593420. DOI: 10.3390/gels10110738.

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