Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of As Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream ()

Overview

Journal Animals (Basel)

Date 2023 Aug 12

PMID 37570345

Authors

Efthimia Antonopoulou

Markos Kolygas

Nikolas Panteli

Evangelia Gouva

Panagiota Kontogeorgiou

Konstantinos Feidantsis

Achilleas Chatzopoulos

Konstantina Bitchava

Christos Zacharis

Eleftherios Bonos

Ilias Giannenas

Ioannis Skoufos

Stefanos S Andreadis

Georgios Skoulakis

Christos G Athanassiou

Cosmas Nathanailides

Affiliations

Soon will be listed here.

Abstract

This work assesses the dietary use of two insect meals of (TM) larvae reared in conventional (TM-10) or MAP-enriched substrates (MAP-TM-10) as fish meal replacements (10%) in the diets of gilthead seabream (). Fish ( = 4500; 207.19 ± 1.47 g) were divided into three groups with triplicates: control (fed conventional diet), TM-10, and MAP-TM-10 groups. The fish were reared in floating cages for 12 weeks and the dietary effects on white blood cell activation, heat shock proteins, MAPKs, and apoptosis of the fish were evaluated. The MAP-TM-10 group exhibited the highest eosinophilic induction. Phosphorylated levels of p38 MAPK, p44/42 MAPK, HSP70, and HSP90 increased in the TM-10 and MAP-TM-10 groups. In terms of apoptosis, Bax levels were lower in the TM groups compared to the control, and the MAP-TM-10 group showed even lower levels than the TM-10 group. Bcl-2 levels increased in the TM-10 group compared to the control, and further increased in the MAP-TM-10 group. The Bax/Bcl-2 ratio, an apoptosis indicator, decreased in the TM groups, with the MAP-TM-10 group showing a further decrease compared to TM-10. These findings suggest that insects' breeding substrate being enriched with MAPs modulated the effect of TM on cellular stress and apoptosis.

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