Co-Existence of , , , and in a Clinical Isolate of from Alexandria, Egypt

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Aug 12

PMID 37569889

Authors

Sandra Sanchez-Urtaza

Alain Ocampo-Sosa

Ainhoa Molins-Bengoetxea

Jorge Rodriguez-Grande

Mohammed A El-Kholy

Marta Hernandez

David Abad

Sherine M Shawky

Itziar Alkorta

Lucia Gallego

Affiliations

Soon will be listed here.

Abstract

The increasing rates of antimicrobial resistance among carbapenem-resistant in the Middle East and North Africa are one of the major concerns for healthcare settings. We characterised the first isolate harbouring five β-lactamases identified in Egypt. The isolate Ale25 was obtained from an ICU patient of a hospital from Alexandria. The isolate was phenotypically and genotypically screened for carbapenemase genes. The isolate was resistant to carbapenems, aminoglycosides, fluoroquinolones and cefiderocol. Whole-Genome Sequencing identified five β-lactamase genes, , , , and , together with other antibiotic resistance genes, conferring resistance to sulfonamides, macrolides, tetracyclines, rifamycin and chloramphenicol. Virulome analysis showed the presence of genes involved in adhesion and biofilm production, type II and VI secretion systems, exotoxins, etc. Multi-Locus Sequence Typing analysis identified the isolate as Sequence Types 113 and 2246, belonging to International Clone 7. Sequencing experiments revealed the presence of four plasmids of 2.7, 22.3, 70.4 and 240.8 Kb. All the β-lactamase genes were located in the chromosome, except the , gene which was found within the plasmid of 240.8 Kb. This study highlights the threat of the emergence and dissemination of these types of isolates.

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