A Comparison of Manual and Electronic Counting for Total Nucleated Cell Counts on Synovial Fluid from Canine Stifle Joints

Overview

Journal Can J Vet Res

Specialty Veterinary Medicine

Date 1986 Apr 1

PMID 3756682

Citations 4

Authors

M A Atilola

J H Lumsden

F Rooke

Affiliations

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Abstract

Synovial fluids collected from the stifle joints of 20 physically normal adult dogs were subjected to cytological examination. A total nucleated cell count was performed on each sample using both an electronic cell counter and a hemocytometer. The mean of the total counts done with the electronic counter was significantly higher (1008 cells/microL) than that obtained manually with the hemocytometer (848 cells/microL).

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References

HOLLANDER J, Reginato A, Torralba T . Examination of synovial fluid as a diagnostic aid in arthritis. Med Clin North Am. 1966; 50(5):1281-93. View

Palmer D . Total leukocyte enumeration in pathologic synovial fluids. Am J Clin Pathol. 1968; 49(6):812-4. DOI: 10.1093/ajcp/49.6.812. View

McCarty Jr D, Phelps P, Pyenson J . Crystal-induced inflammation in canine joints. I. An experimental model with quantification of the host response. J Exp Med. 1966; 124(1):99-114. PMC: 2138217. DOI: 10.1084/jem.124.1.99. View

Pond M . Normal joint tissues and their reaction to injury. Vet Clin North Am. 1971; 1(3):523-35. DOI: 10.1016/s0091-0279(71)50061-2. View

Pedersen N . Synovial fluid collection and analysis. Vet Clin North Am. 1978; 8(3):495-9. DOI: 10.1016/s0091-0279(78)50056-7. View