Ultrasensitive Levofloxacin Electrochemical Biosensor Based on Semiconducting Covalent Organic Framework/poly-L-cysteine/triangular Ag Nanoplates Modified Glassy Carbon Electrode

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2023 Aug 9

PMID 37555996

Authors

Lei Sun

Hao Guo

Bingqing Liu

Zhilan Pan

Ning Wu

Hao Zhang

Wu Yang

Affiliations

Soon will be listed here.

Abstract

A novel electrochemical biosensor with excellent performance was fabricated for levofloxacin (LEV) detection, which adopted triangular Ag nanoplates (Tri-AgNP) confined in a poly-L-cysteine (poly-L-Cys) film and a semiconducting covalent organic framework (COF) as the electrochemical sensing material. The developed electrochemical sensor revealed excellent analytical properties because of its good electrical conductivity, fast electron transfer, and abundant bioactive site. Based on this, a linear relationship between the LEV concentration and the peak current response at 0.92 V was obtained under the optimal experimental conditions by differential pulse voltammetry (DPV), with a wide linear range of 0.05 to 600 μM and a low limit of detection (LOD) of 0.0061 μM. The prepared sensor also realized sensitive and accurate determination of LEV in human serum and urine samples by standard addition method, with satisfactory recoveries (97.1 to 104%) and a low relative standard deviation of less than 4.6%. These results indicated that the novel ternary system has a promising application in the development of electrochemical signal probe and electrochemical biosensing platform.

References

Joshi A, Kim K . Recent advances in nanomaterial-based electrochemical detection of antibiotics: Challenges and future perspectives. Biosens Bioelectron. 2020; 153:112046. DOI: 10.1016/j.bios.2020.112046. View

Faria A, de Souza M, de Almeida M, de Oliveira M . Simultaneous separation of five fluoroquinolone antibiotics by capillary zone electrophoresis. Anal Chim Acta. 2007; 579(2):185-92. DOI: 10.1016/j.aca.2006.07.037. View

Wang J, Lian X, Yan B . Eu-Functionalized Covalent Organic Framework Hybrid Material as a Sensitive Turn-On Fluorescent Switch for Levofloxacin Monitoring in Serum and Urine. Inorg Chem. 2019; 58(15):9956-9963. DOI: 10.1021/acs.inorgchem.9b01106. View

Wu Y, Yan D, Zhang Z, Matsushita M, Awaga K . Electron Highways into Nanochannels of Covalent Organic Frameworks for High Electrical Conductivity and Energy Storage. ACS Appl Mater Interfaces. 2019; 11(8):7661-7665. DOI: 10.1021/acsami.8b21696. View

Keller N, Bein T . Optoelectronic processes in covalent organic frameworks. Chem Soc Rev. 2020; 50(3):1813-1845. DOI: 10.1039/d0cs00793e. View

Ding H, Li J, Xie G, Lin G, Chen R, Peng Z . An AIEgen-based 3D covalent organic framework for white light-emitting diodes. Nat Commun. 2018; 9(1):5234. PMC: 6286360. DOI: 10.1038/s41467-018-07670-4. View

Huang N, Krishna R, Jiang D . Tailor-Made Pore Surface Engineering in Covalent Organic Frameworks: Systematic Functionalization for Performance Screening. J Am Chem Soc. 2015; 137(22):7079-82. DOI: 10.1021/jacs.5b04300. View

Fan H, Mundstock A, Feldhoff A, Knebel A, Gu J, Meng H . Covalent Organic Framework-Covalent Organic Framework Bilayer Membranes for Highly Selective Gas Separation. J Am Chem Soc. 2018; 140(32):10094-10098. DOI: 10.1021/jacs.8b05136. View

Li H, Pan Q, Ma Y, Guan X, Xue M, Fang Q . Three-Dimensional Covalent Organic Frameworks with Dual Linkages for Bifunctional Cascade Catalysis. J Am Chem Soc. 2016; 138(44):14783-14788. DOI: 10.1021/jacs.6b09563. View

10.

Han X, Xia Q, Huang J, Liu Y, Tan C, Cui Y . Chiral Covalent Organic Frameworks with High Chemical Stability for Heterogeneous Asymmetric Catalysis. J Am Chem Soc. 2017; 139(25):8693-8697. DOI: 10.1021/jacs.7b04008. View

11.

Chen L, He L, Ma F, Liu W, Wang Y, Silver M . Covalent Organic Framework Functionalized with 8-Hydroxyquinoline as a Dual-Mode Fluorescent and Colorimetric pH Sensor. ACS Appl Mater Interfaces. 2018; 10(18):15364-15368. DOI: 10.1021/acsami.8b05484. View

12.

Sun Q, Aguila B, Perman J, Earl L, Abney C, Cheng Y . Postsynthetically Modified Covalent Organic Frameworks for Efficient and Effective Mercury Removal. J Am Chem Soc. 2017; 139(7):2786-2793. DOI: 10.1021/jacs.6b12885. View

13.

Li Z, Huang N, Lee K, Feng Y, Tao S, Jiang Q . Light-Emitting Covalent Organic Frameworks: Fluorescence Improving via Pinpoint Surgery and Selective Switch-On Sensing of Anions. J Am Chem Soc. 2018; 140(39):12374-12377. DOI: 10.1021/jacs.8b08380. View

14.

Xu F, Yang S, Chen X, Liu Q, Li H, Wang H . Energy-storage covalent organic frameworks: improving performance engineering polysulfide chains on walls. Chem Sci. 2019; 10(23):6001-6006. PMC: 6566448. DOI: 10.1039/c8sc04518f. View

15.

DeBlase C, Silberstein K, Truong T, Abruna H, Dichtel W . β-Ketoenamine-linked covalent organic frameworks capable of pseudocapacitive energy storage. J Am Chem Soc. 2013; 135(45):16821-4. DOI: 10.1021/ja409421d. View

16.

Liu S, Tao H, Zeng L, Liu Q, Xu Z, Liu Q . Shape-Dependent Electrocatalytic Reduction of CO to CO on Triangular Silver Nanoplates. J Am Chem Soc. 2017; 139(6):2160-2163. DOI: 10.1021/jacs.6b12103. View

17.

Sun Y, Xia Y . Shape-controlled synthesis of gold and silver nanoparticles. Science. 2002; 298(5601):2176-9. DOI: 10.1126/science.1077229. View

18.

Shi R, Liu L, Lu Y, Wang C, Li Y, Li L . Nitrogen-rich covalent organic frameworks with multiple carbonyls for high-performance sodium batteries. Nat Commun. 2020; 11(1):178. PMC: 6954217. DOI: 10.1038/s41467-019-13739-5. View