Intervention Effects and Related Mechanisms of Glycyrrhizic Acid on Zebrafish with Hirschsprung-associated Enterocolitis

Overview

Journal World J Gastrointest Surg

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2023 Aug 9

PMID 37555121

Authors

Ming-Kun Liu

Ying-Jian Chen

Fei Chen

Zhi-Xiong Lin

Zi-Cheng Zhu

Yu Lin

Yi-Fan Fang

Dian-Ming Wu

Affiliations

Soon will be listed here.

Abstract

Background: The prevention and treatment of Hirschsprung-associated enterocolitis (HAEC) is a serious challenge in pediatric surgery. Exploring the mechanism of HAEC is conducive to the prevention of this disease.

Aim: To explore the possible mechanism of glycyrrhizic acid (GA) and its therapeutic effect on HAEC.

Methods: We developed a model of enteritis induced by trinitrobenzenesulfonic acid (TNBS) in zebrafish, and treated it with different concentrations of GA. We analyzed the effect of GA on the phenotype and inflammation of zebrafish.

Results: After treatment with TNBS, the area of the intestinal lumen in zebrafish was significantly increased, but the number of goblet cells in the intestinal lumen was significantly reduced, but these did not increase the mortality of zebrafish, indicating that the zebrafish enteritis model was successfully developed. Different concentrations of GA protected zebrafish with enteritis. In particular, high concentrations of GA were important for the prevention and control of HAEC because it significantly reduced the intestinal luminal area, increased the number of goblet cells in the intestinal lumen, and reduced the levels of interleukin (IL)-1β and IL-8.

Conclusion: GA significantly reduced the intestinal luminal area, increased the number of intestinal goblet cells, and decreased IL-1β and IL-8 in zebrafish, and is important for prevention and control of HAEC.

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