Mini-organs with Big Impact: Organoids in Liver Cancer Studies

Overview

Journal Oncol Res

Specialty Oncology

Date 2023 Aug 7

PMID 37547759

Authors

Muhammad Babar Khawar

Yajun Wang

Aneeqa Majeed

Ali Afzal

Kabeer Haneef

Haibo Sun

Affiliations

Soon will be listed here.

Abstract

Hepatocellular carcinoma, the most common primary liver cancer and a leading cause of death, is a difficult disease to treat due to its heterogeneous nature. Traditional models, such as 2D culture and patient-derived xenografts, have not proven effective. However, the development of 3D culture techniques, such as organoids, which can mimic the tumor microenvironment (TME) and preserve heterogeneity and pathophysiological properties of tumor cells, offers new opportunities for treatment and research. Organoids also have the potential for biomarker detection and personalized medication, as well as genome editing using CRISPR/Cas9 to study the behavior of certain genes and therapeutic interventions. This review explores to-the-date development of organoids with a focus on TME modeling in 3D organoid cultures. Further, it discusses gene editing using CRISPR/Cas9 in organoids, the challenges faced, and the prospects in the field of organoids.

Citing Articles

Tumor organoids for primary liver cancers: A systematic review of current applications in diagnostics, disease modeling, and drug screening.

Qureshi A, Wehrle C, Ferreira-Gonzalez S, Jiao C, Hong H, Dadgar N JHEP Rep. 2024; 6(12):101164.

PMID: 39583095 PMC: 11584567. DOI: 10.1016/j.jhepr.2024.101164.

Primary liver cancer organoids and their application to research and therapy.

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Organoid as a promising tool for primary liver cancer research: a comprehensive review.

Hu X, Wei J, Liu P, Zheng Q, Zhang Y, Zhang Q Cell Biosci. 2024; 14(1):107.

PMID: 39192365 PMC: 11348559. DOI: 10.1186/s13578-024-01287-5.

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