The Effect of The Conditioned Medium from Human Embryonic Stem Cells on Mouse Oocytes In Vitro Maturation

Overview

Journal Cell J

Specialty Cell Biology

Date 2023 Aug 6

PMID 37543858

Authors

Roshan Rezaee Ranjbar Sardari

Mahmood Dehghani Ashkezari

Seyed Morteza Seifati

Mahin Izadi

Jalal Golzadeh

Fahime Mazaheri

Maryam Adib

Behrouz Aflatoonian

Affiliations

Soon will be listed here.

Abstract

Objective: Some reports have indicated that conditioned medium from growing mouse embryonic stem cells (ESCs) provides a supportive condition for small follicles growing, oocyte maturation, and following embryo growth. The aim of this study is assessing in vitro maturation (IVM) and consequent fertilization (IVF) outcome of immature mouse oocytes using human embryonic stem cells conditioned medium (HESCM).

Materials And Methods: In this experimental study, 240 germinal vesicle (GV) oocytes were took from NMRI female mice, aged 4-6 weeks, 48 hours before injection of 5 IU pregnant mare serum gonadotropin (PMSG). 120 GV oocytes without cumulus cells were cultured in each of the groups. 120 GV were cultured in HESCM as test groups and also 120 GV cultured in human embryonic stem cells medium (HESM) as control groups. After evaluating the metaphase II (MII) oocyte maturation rate at 8, 16 and 24 hours, the MII oocytes subsequently were fertilized and the two-cell embryo development rate was recorded at days 1, 2, and 3. Statistical analysis was performed by using the generalized estimating equations (GEE) method that calculated their rate ratio.

Results: Our data indicated there are significant differences between the maturation rates in HESCM and HESM (P=0.004), also the two-cell embryo development was significant between two culture media (P=0.00).

Conclusion: Similar to some other studies, the secretome of the HESCM showed a significant impact on the IVM outcomes in mice.

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