Functional Divergence of CYP76AKs Shapes the Chemodiversity of Abietane-type Diterpenoids in Genus Salvia

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Aug 4

PMID 37542034

Authors

Jiadong Hu

Shi Qiu

Feiyan Wang

Qing Li

Chun-Lei Xiang

Peng Di

Ziding Wu

Rui Jiang

Jinxing Li

Zhen Zeng

Jing Wang

Xingxing Wang

Yuchen Zhang

Shiyuan Fang

Yuqi Qiao

Jie Ding

Yun Jiang

Zhichao Xu

Junfeng Chen

Wansheng Chen

Affiliations

Soon will be listed here.

Abstract

The genus Salvia L. (Lamiaceae) comprises myriad distinct medicinal herbs, with terpenoids as one of their major active chemical groups. Abietane-type diterpenoids (ATDs), such as tanshinones and carnosic acids, are specific to Salvia and exhibit taxonomic chemical diversity among lineages. To elucidate how ATD chemical diversity evolved, we carried out large-scale metabolic and phylogenetic analyses of 71 Salvia species, combined with enzyme function, ancestral sequence and chemical trait reconstruction, and comparative genomics experiments. This integrated approach showed that the lineage-wide ATD diversities in Salvia were induced by differences in the oxidation of the terpenoid skeleton at C-20, which was caused by the functional divergence of the cytochrome P450 subfamily CYP76AK. These findings present a unique pattern of chemical diversity in plants that was shaped by the loss of enzyme activity and associated catalytic pathways.

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