Alternative Methods for RuBisCO Extraction from Sugar Beet Waste: A Comparative Approach of Ultrasound and High Voltage Electrical Discharge

Overview

Journal Ultrason Sonochem

Specialty Radiology

Date 2023 Aug 4

PMID 37541125

Authors

Josipa Dukic

Karla Kospic

Vanja Kelava

Renata Mavric

Marinela Nutrizio

Biljana Balen

Ana Butorac

Mecit Halil Oztop

Anet Rezek Jambrak

Affiliations

Soon will be listed here.

Abstract

Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/g with cold and 96.75 ± 4.30 mg/g with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/g with argon and 34.89 ± 1.59 mg/g with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products.

Citing Articles

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References

Zahari I, Ostbring K, Purhagen J, Rayner M . Plant-Based Meat Analogues from Alternative Protein: A Systematic Literature Review. Foods. 2022; 11(18). PMC: 9498552. DOI: 10.3390/foods11182870. View

McClements D, Grossmann L . The science of plant-based foods: Constructing next-generation meat, fish, milk, and egg analogs. Compr Rev Food Sci Food Saf. 2021; 20(4):4049-4100. DOI: 10.1111/1541-4337.12771. View

Zuntar I, Putnik P, Bursac Kovacevic D, Nutrizio M, Supljika F, Poljanec A . Phenolic and Antioxidant Analysis of Olive Leaves Extracts ( L.) Obtained by High Voltage Electrical Discharges (HVED). Foods. 2019; 8(7). PMC: 6678916. DOI: 10.3390/foods8070248. View

Yasui K, Tuziuti T, Sivakumar M, Iida Y . Theoretical study of single-bubble sonochemistry. J Chem Phys. 2005; 122(22):224706. DOI: 10.1063/1.1925607. View

Pimentel D, Pimentel M . Sustainability of meat-based and plant-based diets and the environment. Am J Clin Nutr. 2003; 78(3 Suppl):660S-663S. DOI: 10.1093/ajcn/78.3.660S. View

Kaur L, Lamsar H, Lopez I, Filippi M, Ong Shu Min D, Ah-Sing K . Physico-Chemical Characteristics and In Vitro Gastro-Small Intestinal Digestion of New Zealand Ryegrass Proteins. Foods. 2021; 10(2). PMC: 7913788. DOI: 10.3390/foods10020331. View

Tiban N, Simovic M, Polovic M, Saric A, Tomac I, Matic P . The Effect of High Voltage Electrical Discharge on the Physicochemical Properties and the Microbiological Safety of Rose Hip Nectars. Foods. 2022; 11(5). PMC: 8909758. DOI: 10.3390/foods11050651. View

Bakhsh A, Lee S, Lee E, Hwang Y, Joo S . Evaluation of Rheological and Sensory Characteristics of Plant-Based Meat Analog with Comparison to Beef and Pork. Food Sci Anim Resour. 2021; 41(6):983-996. PMC: 8564321. DOI: 10.5851/kosfa.2021.e50. View

Chevallet M, Diemer H, Van Dorssealer A, Villiers C, Rabilloud T . Toward a better analysis of secreted proteins: the example of the myeloid cells secretome. Proteomics. 2007; 7(11):1757-70. PMC: 2386146. DOI: 10.1002/pmic.200601024. View

10.

Neacsu M, Raikos V, Benavides-Paz Y, Duncan S, Duncan G, Christie J . Sapogenol is a Major Microbial Metabolite in Human Plasma Associated with High Protein Soy-Based Diets: The Relevance for Functional Food Formulations. Foods. 2020; 9(4). PMC: 7230228. DOI: 10.3390/foods9040422. View

11.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

12.

Yao S, Li W, Wu Y, Martin G, Ashokkumar M . The Impact of High-Intensity Ultrasound-Assisted Extraction on the Structural and Functional Properties of Hempseed Protein Isolate (HPI). Foods. 2023; 12(2). PMC: 9858475. DOI: 10.3390/foods12020348. View

13.

Yasui K, Tuziuti T, Kozuka T, Towata A, Iida Y . Relationship between the bubble temperature and main oxidant created inside an air bubble under ultrasound. J Chem Phys. 2007; 127(15):154502. DOI: 10.1063/1.2790420. View

14.

Rahman M, Lamsal B . Ultrasound-assisted extraction and modification of plant-based proteins: Impact on physicochemical, functional, and nutritional properties. Compr Rev Food Sci Food Saf. 2021; 20(2):1457-1480. DOI: 10.1111/1541-4337.12709. View

15.

Vermeulen S, Park T, Khoury C, Bene C . Changing diets and the transformation of the global food system. Ann N Y Acad Sci. 2020; 1478(1):3-17. PMC: 7689688. DOI: 10.1111/nyas.14446. View

16.

Bonke A, Sieuwerts S, Petersen I . Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea. Foods. 2020; 9(4). PMC: 7230155. DOI: 10.3390/foods9040429. View

17.

Carpentier S, Witters E, Laukens K, Deckers P, Swennen R, Panis B . Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two-dimensional gel electrophoresis analysis. Proteomics. 2005; 5(10):2497-507. DOI: 10.1002/pmic.200401222. View

18.

Zocca F, Lomolino G, Lante A . Antibrowning potential of Brassicacaea processing water. Bioresour Technol. 2010; 101(10):3791-5. DOI: 10.1016/j.biortech.2009.12.126. View

19.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

20.

Carmo-Silva A, Barta C, Salvucci M . Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves. Methods Mol Biol. 2010; 684:339-47. DOI: 10.1007/978-1-60761-925-3_26. View