Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias

Overview

Journal Avicenna J Med Biotechnol

Specialty Biomedical Engineering

Date 2023 Aug 4

PMID 37538244

Authors

Parastou Gholami

Hossein Asgarian-Omran

Marjan Yaghmaie

Jafar Mahmudian

Shirin Kianersi

Sina Salari

Ehsan Zaboli

Mahmood Jeddi-Tehrani

Amir-Hassan Zarnani

Mahdi Shabani

Affiliations

Soon will be listed here.

Abstract

Background: Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL).

Methods: In this study, we investigated expression pattern of gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting.

Results: Our data demonstrated that transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. transcript expression was totally negative in all studied normal subjects. Level of mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of in AML subtypes was not statistically significant (p=0.756). gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples.

Conclusion: Considering expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future.

Citing Articles

Identification of placenta-specific protein 1 (PLAC-1) expression on human PC-3 cell line-derived prostate cancer stem cells compared to the tumor parental cells.

Farhangnia P, Ghods R, Falak R, Zarnani A, Delbandi A Discov Oncol. 2024; 15(1):251.

PMID: 38943028 PMC: 11213845. DOI: 10.1007/s12672-024-01121-x.

Placenta: an old organ with new functions.

Khorami-Sarvestani S, Vanaki N, Shojaeian S, Zarnani K, Stensballe A, Jeddi-Tehrani M Front Immunol. 2024; 15:1385762.

PMID: 38707901 PMC: 11066266. DOI: 10.3389/fimmu.2024.1385762.

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