Characterization of a Novel Interaction of the Nup159 Nucleoporin with Asymmetrically Localized Spindle Pole Body Proteins and Its Link with Autophagy

Overview

Journal PLoS Biol

Specialty Biology

Date 2023 Aug 3

PMID 37535687

Authors

Ines Garcia de Oya

Javier Manzano-Lopez

Alejandra Alvarez-Llamas

Maria de la Paz Vazquez-Aroca

Cristina Cepeda-Garcia

Fernando Monje-Casas

Affiliations

Soon will be listed here.

Abstract

Both the spindle microtubule-organizing centers and the nuclear pore complexes (NPCs) are convoluted structures where many signaling pathways converge to coordinate key events during cell division. Interestingly, despite their distinct molecular conformation and overall functions, these structures share common components and collaborate in the regulation of essential processes. We have established a new link between microtubule-organizing centers and nuclear pores in budding yeast by unveiling an interaction between the Bfa1/Bub2 complex, a mitotic exit inhibitor that localizes on the spindle pole bodies, and the Nup159 nucleoporin. Bfa1/Bub2 association with Nup159 is reduced in metaphase to not interfere with proper spindle positioning. However, their interaction is stimulated in anaphase and assists the Nup159-dependent autophagy pathway. The asymmetric localization of Bfa1/Bub2 during mitosis raises the possibility that its interaction with Nup159 could differentially promote Nup159-mediated autophagic processes, which might be relevant for the maintenance of the replicative lifespan.

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