The Transcription Factor Masculinizer in Sexual Differentiation and Achieved Full Functional Sex Reversal in Prawn

Overview

Journal iScience

Publisher Cell Press

Date 2023 Aug 3

PMID 37534170

Authors

Jing-Yu Luo

Shuai-Qi Shen

Hai-Jing Xu

Jin-Shu Yang

Wen-Ming Ma

Affiliations

Soon will be listed here.

Abstract

Some Zinc finger (ZnF) proteins are required for masculinization in silkworms. In the present study, a masculinizer gene (Mr-Masc) with multi-tissue expression is identified in the freshwater prawn . The Mr-Masc is clustered into a separate branch with ZnF proteins from decapoda by phylogenetic tree analysis. Moreover, Mr-Masc silencing in male postlarvae prawn results in functional sex reversal females known as neo-females, which are applied to all-male monosex offspring breeding. This manipulation has been significant in sexually dimorphic cultured species. In addition, several significantly expressed transcripts are enriched and the effects of crucial signal pathways are focused through the comparative transcriptomic analysis in Mr-Masc gene knockdown. The significantly differentially expressed epidermal growth factor, upregulated low-density lipoprotein receptor, flotillin, and sex-lethal unigenes, downregulated heat shock proteins and forkhead box homologs are focused. The finding offers an innovative perspective on Masc proteins' evolution and physiological function.

Citing Articles

Monosex Populations of the Giant Freshwater Prawn -From a Pre-Molecular Start to the Next Generation Era.

Wahl M, Levy T, Ventura T, Sagi A Int J Mol Sci. 2023; 24(24).

PMID: 38139271 PMC: 10743721. DOI: 10.3390/ijms242417433.

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