Quercetin Ameliorates Neuropathic Pain After Brachial Plexus Avulsion Suppressing Oxidative Damage Through Inhibition of PKC/MAPK/ NOX Pathway

Overview

Journal Curr Neuropharmacol

Publisher Bentham Science Publishers

Date 2023 Aug 3

PMID 37533160

Authors

Yanfeng Huang

Xie Zhang

Yidan Zou

Qiuju Yuan

Yan-Fang Xian

Zhi-Xiu Lin

Affiliations

Soon will be listed here.

Abstract

Background: Brachial plexus avulsion (BPA) animally involves the separation of spinal nerve roots themselves and the correlative spinal cord segment, leading to formidable neuropathic pain of the upper limb.

Methods: The right seventh cervical (C7) ventral and dorsal roots were avulsed to establish a neuropathic pain model in rats. After operation, rats were treated with quercetin (QCN) by intragastric administration for 1 week. The effects of QCN were evaluated using mechanical allodynia tests and biochemical assay kits.

Results: QCN treatment significantly attenuated the avulsion-provoked mechanical allodynia, elevated the levels of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) and total antioxidant capacity (TAC) in the C7 spinal dorsal horn. In addition, QCN administration inhibited the activations of macrophages, microglia and astrocytes in the C6 dorsal root ganglion (DRG) and C6-8 spinal dorsal horn, as well as attenuated the release of purinergic 2X (P2X) receptors in C6 DRG. The molecular mechanism underlying the above alterations was found to be related to the suppression of the PKC/MAPK/NOX signal pathway. To further study the anti-oxidative effects of QCN, we applied QCN on the HO-induced BV-2 cells , and the results attested that QCN significantly ameliorated the HO-induced ROS production in BV-2 cells, inhibited the HO-induced activation of PKC/MAPK/NOX pathway.

Conclusion: Our study for the first time provided evidence that QCN was able to attenuate pain hypersensitivity following the C7 spinal root avulsion in rats, and the molecular mechanisms involve the reduction of both neuro-inflammatory infiltration and oxidative stress via suppression of P2X receptors and inhibition of the activation of PKC/MAPK/NOX pathway. The results indicate that QCN is a natural compound with great promise worthy of further development into a novel therapeutic method for the treatment of BPA-induced neuropathic pain.

Citing Articles

A comprehensive review of traditional Chinese medicine in treating neuropathic pain.

Hu N, Liu J, Luo Y, Li Y Heliyon. 2024; 10(17):e37350.

PMID: 39296122 PMC: 11407996. DOI: 10.1016/j.heliyon.2024.e37350.

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