Nuclear Transfer Improves the Developmental Potential of Embryos Derived from Cytoplasmic Deficient Oocytes

Overview

Journal iScience

Publisher Cell Press

Date 2023 Jul 31

PMID 37520712

Authors

Zhaodi Liao

Yuzhuo Li

Chunyang Li

Xinyan Bian

Qiang Sun

Affiliations

Soon will be listed here.

Abstract

Embryo development after fertilization is largely determined by the oocyte quality, which is in turn dependent on the competence of both the cytoplasm and nucleus. Here, to improve the efficiency of embryo development from developmentally incompetent oocytes, we performed spindle-chromosome complex transfer (ST) between matured (IVM) and matured (IVO) oocytes of the non-human primate rhesus monkey. We observed that the blastocyst rate of embryos derived from transferring the spindle-chromosome complex (SCC) of IVM oocytes into enucleated IVO oocytes was comparable with that of embryos derived from IVO oocytes. After transferring the reconstructed embryos into the uterus of surrogate mothers, two live rhesus monkeys were obtained, indicating that the nuclei of IVM oocytes support both the pre-and post-implantation embryo development of non-human primates.

Citing Articles

Cumulus cell DNA damage linked to fertilization success in females with an ovulatory dysfunction phenotype.

Rodrigues B, Sousa V, Esteves F, Vale-Fernandes E, Costa S, Sousa D Front Cell Dev Biol. 2024; 12:1448733.

PMID: 39605981 PMC: 11599247. DOI: 10.3389/fcell.2024.1448733.

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