Function Discovery of a Non-ribosomal Peptide Synthetase-like Encoding Gene in the Nematode-trapping Fungus

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Jul 31

PMID 37520361

Authors

Tiantian Gu

Hengqian Lu

Huiwen Liu

Guanghui Zhang

Yongzhong Wang

Affiliations

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Abstract

In this study, the function of a non-ribosomal peptide synthetase-like (NRPS-like) encoding gene () was investigated to reveal the association between NRPS and nematocidal activity in the nematode-trapping fungus . Sequence analysis indicated that the encoded product of is an adenylation domain of non-ribosomal peptide synthetases and extended short-chain dehydrogenase/reductase domain-containing proteins, and displays a wide substrate spectrum. The Δ mutants were more sensitive to chemical stressors than the wild type. Disruption of impeded the nematocidal efficiency of . Metabolomics analysis showed that secondary metabolite biosynthesis and lipid metabolism were altered in the mutants. The phenotypic changes in the mutants can be attributed to the down-regulation of various metabolites, including fatty acyls, prenol lipids, steroidsand steroid derivative, and amino acid derivatives, identified in the present study. This study investigated the association between the non-ribosomal polypeptide-encoding gene and nematicidal activity in , providing a reference for resolving the predation mechanism of nematode-trapping fungus.

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