Iron-Coordinated L-Lysine-Based Nanozymes with High Peroxidase-like Activity for Sensitive Hydrogen Peroxide and Glucose Detection

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2023 Jul 29

PMID 37514392

Authors

Xiuqing Hou

Ruoxue Wang

Huijuan Zhang

Meng Zhang

Xiongwei Qu

Xiuli Hu

Affiliations

Soon will be listed here.

Abstract

It is crucial to develop sensitive and accurate sensing strategies to detect HO and glucose in biological systems. Herein, biocompatible iron-coordinated L-lysine-based hydrogen peroxide (HO)-mimetic enzymes (Lys-Fe-NPs) were prepared by precipitation polymerization in aqueous solution. The coordinated Fe ion acted as centers of peroxidase-like enzymes of Lys-Fe-NPs, and the catalytic activity was evaluated via the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO. Therefore, a sensitive colorimetric detection sensor for HO was constructed with a linear range of 1 to 200 μM and a detection limit of 0.51 μM. The same method could also be applied to highly sensitive and selective detection of glucose, with a linear range of 0.5 to 150 μM and a detection limit of 0.32 μM. In addition, an agarose-based hydrogel biosensor colorimetric was successfully implemented for visual assessment and quantitative detection of glucose. The design provided a novel platform for constructing stable and nonprotein enzyme mimics with lysine and showed great potential applications in biorelevant assays.

Citing Articles

Iron-Based Nanoplatforms Achieve Hepatocellular Carcinoma Regression Through a Cascade of Effects.

Huang K, Yi X, Xie H, Luo J, Zeng Q, He F Int J Nanomedicine. 2024; 19:11105-11128.

PMID: 39502633 PMC: 11537158. DOI: 10.2147/IJN.S479425.

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