Fisetin Delays Postovulatory Oocyte Aging by Regulating Oxidative Stress and Mitochondrial Function Through Pathway

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Jul 29

PMID 37513404

Authors

Xupeng Xing

Yalin Liang

Yanan Li

Yaolu Zhao

Yuxing Zhang

Zheng Li

Zicong Li

Zhenfang Wu

Affiliations

Soon will be listed here.

Abstract

The quality of oocytes determines the development potential of an embryo and is dependent on their timely fertilization after ovulation. Postovulatory oocyte aging is an inevitable factor during some assisted reproduction technology procedures, which results in poor fertilization rates and impairs embryo development. We found that fisetin, a bioactive flavonol contained in fruits and vegetables, delayed postovulatory oocyte aging in mice. Fisetin improved the development of aged oocytes after fertilization and inhibited the reduction in aged oocytes. Fisetin increased the GSH level and transcription level to inhibit ROS accumulation in aged oocytes. Meanwhile, fisetin attenuated aging-induced spindle abnormalities, mitochondrial dysfunction, and apoptosis. At the molecular level, fisetin decreased aging-induced aberrant expression of H3K9me3. In addition, fisetin increased the expression levels of the mitochondrial transcription factor and the mitochondrial genes and by upregulating in aged oocytes. Finally, inhibition of reversed the anti-aging effects of fisetin. Taken together, fisetin delayed postovulatory oocyte aging by upregulating .

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