Lipid Corona Formation on Micro- and Nanoplastic Particles Modulates Uptake and Toxicity in A549 Cells

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2023 Jul 29

PMID 37512356

Authors

Anna Daniela Dorsch

Walison Augusto da Silva Brito

Mihaela Delcea

Kristian Wende

Sander Bekeschus

Affiliations

Soon will be listed here.

Abstract

Plastic waste is a global issue leaving no continents unaffected. In the environment, ultraviolet radiation and shear forces in water and land contribute to generating micro- and nanoplastic particles (MNPP), which organisms can easily take up. Plastic particles enter the human food chain, and the accumulation of particles within the human body is expected. Crossing epithelial barriers and cellular uptake of MNPP involves the interaction of plastic particles with lipids. To this end, we generated unilamellar vesicles from POPC (1-palmitoyl-2-oleoyl-glycero-3-phosphocholine) and POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine) and incubated them with pristine, carboxylated, or aminated polystyrene spheres (about 1 µm in diameter) to generate lipid coronas around the particles. Lipid coronas enhanced the average particle sizes and partially changed the MNPP zeta potential and polydispersity. In addition, lipid coronas led to significantly enhanced uptake of MNPP particles but not their cytotoxicity, as determined by flow cytometry. Finally, adding proteins to lipid corona nanoparticles further modified MNPP uptake by reducing the uptake kinetics, especially in pristine and carboxylated plastic samples. In conclusion, our study demonstrates for the first time the impact of different types of lipids on differently charged MNPP particles and the biological consequences of such modifications to better understand the potential hazards of plastic exposure.

Citing Articles

Advanced Science and Technology of Polymer Matrix Nanomaterials.

Liu P, Xu L, Li J, Peng J, Jiao Z Materials (Basel). 2024; 17(2).

PMID: 38255627 PMC: 10821458. DOI: 10.3390/ma17020461.

References

Qu M, Miao L, Chen H, Zhang X, Wang Y . SKN-1/Nrf2-dependent regulation of mitochondrial homeostasis modulates transgenerational toxicity induced by nanoplastics with different surface charges in Caenorhabditis elegans. J Hazard Mater. 2023; 457:131840. DOI: 10.1016/j.jhazmat.2023.131840. View

Alimi O, Farner Budarz J, Hernandez L, Tufenkji N . Microplastics and Nanoplastics in Aquatic Environments: Aggregation, Deposition, and Enhanced Contaminant Transport. Environ Sci Technol. 2017; 52(4):1704-1724. DOI: 10.1021/acs.est.7b05559. View

Ostle C, Thompson R, Broughton D, Gregory L, Wootton M, Johns D . The rise in ocean plastics evidenced from a 60-year time series. Nat Commun. 2019; 10(1):1622. PMC: 6467903. DOI: 10.1038/s41467-019-09506-1. View

Wanner P . Plastic in agricultural soils - A global risk for groundwater systems and drinking water supplies? - A review. Chemosphere. 2020; 264(Pt 1):128453. DOI: 10.1016/j.chemosphere.2020.128453. View

Singh M, Kumar V, Sikka K, Thakur R, Harioudh M, Mishra D . Computational Design of Biologically Active Anticancer Peptides and Their Interactions with Heterogeneous POPC/POPS Lipid Membranes. J Chem Inf Model. 2019; 60(1):332-341. DOI: 10.1021/acs.jcim.9b00348. View

Rhodes C . Solving the plastic problem: From cradle to grave, to reincarnation. Sci Prog. 2019; 102(3):218-248. PMC: 10424522. DOI: 10.1177/0036850419867204. View

Cao J, Yang Q, Jiang J, Dalu T, Kadushkin A, Singh J . Coronas of micro/nano plastics: a key determinant in their risk assessments. Part Fibre Toxicol. 2022; 19(1):55. PMC: 9356472. DOI: 10.1186/s12989-022-00492-9. View

Pan J, Cheng X, Monticelli L, Heberle F, Kucerka N, Tieleman D . The molecular structure of a phosphatidylserine bilayer determined by scattering and molecular dynamics simulations. Soft Matter. 2014; 10(21):3716-25. DOI: 10.1039/c4sm00066h. View

Yang H, Zhou M, Li H, Wei T, Tang C, Zhou Y . Effects of Low-level Lipid Peroxidation on the Permeability of Nitroaromatic Molecules across a Membrane: A Computational Study. ACS Omega. 2020; 5(10):4798-4806. PMC: 7081259. DOI: 10.1021/acsomega.9b03462. View

10.

Wheeler K, Chetwynd A, Fahy K, Hong B, Tochihuitl J, Foster L . Environmental dimensions of the protein corona. Nat Nanotechnol. 2021; 16(6):617-629. DOI: 10.1038/s41565-021-00924-1. View

11.

Jurkiewicz P, Cwiklik L, Vojtiskova A, Jungwirth P, Hof M . Structure, dynamics, and hydration of POPC/POPS bilayers suspended in NaCl, KCl, and CsCl solutions. Biochim Biophys Acta. 2011; 1818(3):609-16. DOI: 10.1016/j.bbamem.2011.11.033. View

12.

Xiao Q, Zoulikha M, Qiu M, Teng C, Lin C, Li X . The effects of protein corona on in vivo fate of nanocarriers. Adv Drug Deliv Rev. 2022; 186:114356. DOI: 10.1016/j.addr.2022.114356. View

13.

Abdelkhaliq A, van der Zande M, Punt A, Helsdingen R, Boeren S, Vervoort J . Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport. J Nanobiotechnology. 2018; 16(1):70. PMC: 6138932. DOI: 10.1186/s12951-018-0394-6. View

14.

Saptarshi S, Duschl A, Lopata A . Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. J Nanobiotechnology. 2013; 11:26. PMC: 3720198. DOI: 10.1186/1477-3155-11-26. View

15.

Sonnino S, Chiricozzi E, Grassi S, Mauri L, Prioni S, Prinetti A . Gangliosides in Membrane Organization. Prog Mol Biol Transl Sci. 2018; 156:83-120. DOI: 10.1016/bs.pmbts.2017.12.007. View

16.

Jasinski J, Wilde M, Voelkl M, Jerome V, Frohlich T, Freitag R . Tailor-Made Protein Corona Formation on Polystyrene Microparticles and its Effect on Epithelial Cell Uptake. ACS Appl Mater Interfaces. 2022; 14(41):47277-47287. DOI: 10.1021/acsami.2c13987. View

17.

Rhodes C . Plastic pollution and potential solutions. Sci Prog. 2018; 101(3):207-260. PMC: 10365157. DOI: 10.3184/003685018X15294876706211. View

18.

Hartmann N, Huffer T, Thompson R, Hassellov M, Verschoor A, Daugaard A . Are We Speaking the Same Language? Recommendations for a Definition and Categorization Framework for Plastic Debris. Environ Sci Technol. 2019; 53(3):1039-1047. DOI: 10.1021/acs.est.8b05297. View

19.

Bangham A, HORNE R . NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE. J Mol Biol. 1964; 8:660-8. DOI: 10.1016/s0022-2836(64)80115-7. View

20.

Paul M, Stock V, Cara-Carmona J, Lisicki E, Shopova S, Fessard V . Micro- and nanoplastics - current state of knowledge with the focus on oral uptake and toxicity. Nanoscale Adv. 2022; 2(10):4350-4367. PMC: 9417819. DOI: 10.1039/d0na00539h. View