Localization of Insertion Sequences in Plasmids for L-Cysteine Production in

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Jul 29

PMID 37510222

Authors

Kevin Heieck

Thomas Bruck

Affiliations

Soon will be listed here.

Abstract

Insertion sequence elements (ISE) are often found to be responsible for the collapse of production in synthetically engineered . By the transposition of ISE into the open reading frame of the synthetic pathway, cells gain selection advantage over cells expressing the metabolic burdensome production genes. Here, we present the exact entry sites of insertion sequence (IS) families and within plasmids for l-cysteine production in evolved populations. Furthermore, we identified an uncommon occurrence of an 8-bp direct repeat of IS5 which is atypical for this particular family, potentially indicating a new IS5 target site.

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