Effects of Progestin on Modulation of the Expression of Biomarkers in Endometriosis

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2023 Jul 29

PMID 37509675

Authors

Daniela Roxana Matasariu

Alexandra Irma Gabriela Bausic

Cristina Elena Mandici

Iuliana Elena Bujor

Alexandra Elena Cristofor

Elvira Bratila

Ludmila Lozneanu

Lucian Vasile Boiculese

Mihaela Grigore

Alexandra Ursache

Affiliations

Soon will be listed here.

Abstract

Background: Our study aimed to examine the osteopontin (OPN) serum levels and tissue expression of CD44 and OPN in endometriosis-affected women both undergoing and not undergoing progestin treatment, and also to determine their involvement in the pathogenesis of endometriosis.

Methods: Using an ELISA kit, we evaluated the OPN serum levels of healthy and endometriosis-affected women both undergoing and not undergoing progestin treatment. Immunohistochemical (IHC) analyses were used to assess the endometriotic tissue expressions of CD44 and OPN.

Results: There were statistically significant higher OPN serum levels in the healthy control group compared to the women with endometriosis. Furthermore, there were higher OPN serum levels in the endometriosis-affected women undergoing the progestin treatment, but the difference did not reach statistical significance. In comparison to OPN, CD44 expression was significantly higher in all the endometriotic tissue glands and stroma, regardless of the patient's treatment status. Compared to the group receiving therapy, the OPN levels were higher in the endometriosis group not receiving therapy. OPN's robust cytoplasmic expression seemed to be associated with the non-treatment group.

Conclusion: Endometriosis, CD44, and OPN appear to be closely related. This study suggests that endometriosis that has not been treated has an immunological profile distinct to endometriosis that has received treatment.

References

Surrey E, Soliman A, Johns B, Vora J, Taylor H, Agarwal S . Real-World Characterization of Women with Diagnosed Endometriosis Initiating Therapy with Elagolix Using a US Claims Database. Clinicoecon Outcomes Res. 2020; 12:473-479. PMC: 7456657. DOI: 10.2147/CEOR.S264905. View

Niiro E, Kawahara N, Yamada Y, Yoshimoto C, Shimada K, Sudo T . Immunohistochemical expression of CD44v9 and 8-OHdG in ovarian endometrioma and the benign endometriotic lesions adjacent to clear cell carcinoma. J Obstet Gynaecol Res. 2019; 45(11):2260-2266. DOI: 10.1111/jog.14093. View

Fu X, Yao M, Ye C, Fang T, Wu R . Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway : Osteopontin Regulates Endometrial Cell Migration in Endometriosis. Reprod Sci. 2020; 28(2):435-446. PMC: 7808973. DOI: 10.1007/s43032-020-00301-8. View

Lin E, Xi W, Aggarwal N, Shinohara M . Osteopontin (OPN)/SPP1: from its biochemistry to biological functions in the innate immune system and the central nervous system (CNS). Int Immunol. 2022; 35(4):171-180. PMC: 10071791. DOI: 10.1093/intimm/dxac060. View

DAmico F, Skarmoutsou E, Quaderno G, Malaponte G, La Corte C, Scibilia G . Expression and localisation of osteopontin and prominin-1 (CD133) in patients with endometriosis. Int J Mol Med. 2013; 31(5):1011-6. PMC: 3658602. DOI: 10.3892/ijmm.2013.1325. View

Cho S, Ahn Y, Choi Y, Seo S, Nam A, Kim H . Endometrial osteopontin mRNA expression and plasma osteopontin levels are increased in patients with endometriosis. Am J Reprod Immunol. 2009; 61(4):286-93. DOI: 10.1111/j.1600-0897.2009.00692.x. View

Kiyama R . Nutritional implications of ginger: chemistry, biological activities and signaling pathways. J Nutr Biochem. 2020; 86:108486. DOI: 10.1016/j.jnutbio.2020.108486. View

Mignemi G, Facchini C, Raimondo D, Montanari G, Ferrini G, Seracchioli R . A case report of nasal endometriosis in a patient affected by Behcet's disease. J Minim Invasive Gynecol. 2012; 19(4):514-6. DOI: 10.1016/j.jmig.2012.03.005. View

Neto J, Kho R, Siufi D, Baracat E, Anderson K, Abrao M . Cellular, histologic, and molecular changes associated with endometriosis and ovarian cancer. J Minim Invasive Gynecol. 2013; 21(1):55-63. DOI: 10.1016/j.jmig.2013.07.021. View

10.

Poncelet C, Leblanc M, Walker-Combrouze F, Soriano D, Feldmann G, Madelenat P . Expression of cadherins and CD44 isoforms in human endometrium and peritoneal endometriosis. Acta Obstet Gynecol Scand. 2002; 81(3):195-203. DOI: 10.1034/j.1600-0412.2002.810302.x. View

11.

Konno R, Fujiwara H, Netsu S, Odagiri K, Shimane M, Nomura H . Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol. 2007; 58(4):330-43. DOI: 10.1111/j.1600-0897.2007.00507.x. View

12.

Kurose S, Nakayama K, Razia S, Ishikawa M, Ishibashi T, Yamashita H . Whole-Exome Sequencing of Rare Site Endometriosis-Associated Cancer. Diseases. 2021; 9(1). PMC: 7931088. DOI: 10.3390/diseases9010014. View

13.

Gadducci A, Multinu F, Cosio S, Carinelli S, Ghioni M, Aletti G . Clear cell carcinoma of the ovary: Epidemiology, pathological and biological features, treatment options and clinical outcomes. Gynecol Oncol. 2021; 162(3):741-750. DOI: 10.1016/j.ygyno.2021.06.033. View

14.

El-Sahwi K, Bellone S, Cocco E, Casagrande F, Bellone M, Abu-Khalaf M . Overexpression of EpCAM in uterine serous papillary carcinoma: implications for EpCAM-specific immunotherapy with human monoclonal antibody adecatumumab (MT201). Mol Cancer Ther. 2010; 9(1):57-66. PMC: 2806489. DOI: 10.1158/1535-7163.MCT-09-0675. View

15.

Soliman A, Coyne K, Zaiser E, Castelli-Haley J, Fuldeore M . The burden of endometriosis symptoms on health-related quality of life in women in the United States: a cross-sectional study. J Psychosom Obstet Gynaecol. 2017; 38(4):238-248. DOI: 10.1080/0167482X.2017.1289512. View

16.

Vercellini P, Buggio L, Berlanda N, Barbara G, Somigliana E, Bosari S . Estrogen-progestins and progestins for the management of endometriosis. Fertil Steril. 2016; 106(7):1552-1571.e2. DOI: 10.1016/j.fertnstert.2016.10.022. View

17.

Kim H, Yang K, Kang I, Koong M, Kim H, Zhang X . Expression of CD44s, vascular endothelial growth factor, matrix metalloproteinase-2 and Ki-67 in peritoneal, rectovaginal and ovarian endometriosis. J Reprod Med. 2007; 52(3):207-13. View

18.

Giudice L, Kao L . Endometriosis. Lancet. 2004; 364(9447):1789-99. DOI: 10.1016/S0140-6736(04)17403-5. View

19.

Brichant G, Laraki I, Henry L, Munaut C, Nisolle M . New Therapeutics in Endometriosis: A Review of Hormonal, Non-Hormonal, and Non-Coding RNA Treatments. Int J Mol Sci. 2021; 22(19). PMC: 8508913. DOI: 10.3390/ijms221910498. View

20.

Mehdizadehkashi A, Tahermanesh K, Anvari-Yazdi A, Chaichian S, Azarpira N, Nobakht M . Ultrastructural Investigation of Pelvic Peritoneum in Patients With Chronic Pelvic Pain and Subtle Endometriosis in Association With Chromoendoscopy. J Minim Invasive Gynecol. 2016; 24(1):114-123. DOI: 10.1016/j.jmig.2016.09.013. View