Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2023 Jul 29

PMID 37508224

Authors

Micaela Guidotti-Takeuchi

Roberta Torres de Melo

Ligia Nunes de Morais Ribeiro

Carolyne Ferreira Dumont

Rosanne Aparecida Capanema Ribeiro

Barbara de Araujo Brum

Tanaje Luiz Izidio Ferreira de Amorim Junior

Daise Aparecida Rossi

Affiliations

Soon will be listed here.

Abstract

Horizontal gene transfer (HGT) in food matrices has been investigated under conditions that favor gene exchange. However, the major challenge lies in determining the specific conditions pertaining to the adapted microbial pairs associated with the food matrix. HGT is primarily responsible for enhancing the microbial repertoire for the evolution and spread of antimicrobial resistance and is a major target for controlling pathogens of public health concern in food ecosystems. In this study, we investigated Heidelberg (SH) and (EC) regarding gene exchange under conditions mimicking the industrial environment, with the coproducts whey (SL) and chicken juice (CJ). The Heidelberg strain was characterized by antibiotic susceptibility standards and PCR to detect the gene. A concentration of 0.39 mg/mL was determined to evaluate the anti-conjugation activity of nanostructured lipid nanocarriers (NLCs) of essential oils to mitigate β-lactam resistance gene transfer. The results showed that the addition of these coproducts promoted an increase of more than 3.5 (whey) and 2.5 (chicken juice) orders of magnitude in the conjugation process ( < 0.01), and NLCs of sage essential oil significantly reduced the conjugation frequency (CF) by 74.90, 90.6, and 124.4 times when compared to the transfers in the absence of coproducts and the presence of SL and CJ, respectively. For NLCs from olibanum essential oil, the decrease was 4.46-fold for conjugations without inhibitors and 3.12- and 11.3-fold in the presence of SL and CJ. NLCs associated with sage and olibanum essential oils effectively control the transfer of antibiotic resistance genes and are a promising alternative for use at industrial levels.

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