Organization, Replication and Modification of the Human Genome: Temporal Order of Synthesis and Methylation of Two Classes of HeLa NDNA Separated in Ag+--Cs2-SO4 Gradients

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 1979 Feb 15

PMID 375061

Citations 2

Authors

T Eremenko

A Granieri

P Volpe

Affiliations

Soon will be listed here.

Abstract

During the HeLa S-phase, DNA was methylated, at 1-hr intervals in isolated nuclei and fractionated in Ag+-Cs2SO4 gradients providing a heavy GC-rich peak and a main light AT-rich peak. Both size and specific methylation of these peaks changed during the nDNA duplicative phase. Replication of the heavy GC-rich nDNA fraction, which contains genes for ribosomal RNA, occurred in early S; in contrast, replication of the main AT-rich nDNA fraction was maximal in late S. Concomitantly, specific methylation of the GC-rich nDNA was maximal in the first part of S, while that of the AT-rich nDNA was maximal in the second part of S. This suggested that genes are replicated and methylated with order during the S-phase.

Citing Articles

Organization, replication and modification of the human genome: synthesis and methylation of palindromic, repeated and unique HeLa nDNA sequences during the S-phase.

Eremenko T, Timofeeva M, Volpe P Mol Biol Rep. 1980; 6(3):131-6.

PMID: 7442657 DOI: 10.1007/BF00775405.

Repair-modification and evolution of the eukaryotic genome organization.

Volpe P, Eremenko T Cell Biophys. 1989; 15(1-2):41-60.

PMID: 2476226 DOI: 10.1007/BF02991578.

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